LOCUS       sequence-122545-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Stachura DL, Svoboda O, Campbell CA, Espin-Palazon R, Lau RP, Zon 
            LI, Bartunek P, Traver D
  TITLE     The zebrafish granulocyte colony-stimulating factors (Gcsfs): 2 
            paralogous cytokines and their roles in hematopoietic development 
            and maintenance.
  JOURNAL   Blood. 2013 Dec 5;122(24):3918-28. doi: 
            10.1182/blood-2012-12-475392. Epub 2013 Oct 15.
  PUBMED    24128862
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2012-12-475392"; journalName: "Blood"; date: 
            "2013-12-5- 5"; volume: "122"; issue: "24"; pages: "3918-28"
COMMENT     Sequence Label: pHis-zfCSF3b
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatatg gctagccacc atcaccatca ccatggtgga
       61 tccactgaac acactctgag agccttgagt ttggccaaga agatcctaaa cgacattcca
      121 gctgtgcagg agctctgcgc acccaacact ggtttgagtt cctccactga ggagttttta
      181 tccagtgagt tccagatccc gacgttgccg ctgctcaaat ctgaggattt aacactggag
      241 gagcgtgtgc agcggatgct catcggtctg gagcttcatc acagagtcct gctgaagctg
      301 ctggagccct gccgcccaga gaacctactg aacgacctcg cagagctgcg cacgctgctc
      361 ctcctccagg tgccctctgc tggtcctcag gtgttgccgc acacacacac tctctcggct
      421 ccactcagcc agttcaagct gcaggtgcgt gtgcgcgcga cactccggca gctgcgcagc
      481 ttcatgcagg acgtgttccg cagcctgcgc tgcatcagtg tgtctcaatg ctaactggat
      541 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      601 gagcaggact gg
//
