LOCUS       sequence-122546-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Stachura DL, Svoboda O, Campbell CA, Espin-Palazon R, Lau RP, Zon 
            LI, Bartunek P, Traver D
  TITLE     The zebrafish granulocyte colony-stimulating factors (Gcsfs): 2 
            paralogous cytokines and their roles in hematopoietic development 
            and maintenance.
  JOURNAL   Blood. 2013 Dec 5;122(24):3918-28. doi: 
            10.1182/blood-2012-12-475392. Epub 2013 Oct 15.
  PUBMED    24128862
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2012-12-475392"; journalName: "Blood"; date: 
            "2013-12-5- 5"; volume: "122"; issue: "24"; pages: "3918-28"
COMMENT     Sequence Label: pHis-zfCSF3b
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             147..164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
       61 tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg gctagccacc atcaccatca ccatggtgga tccactgaac
      181 acactctgag agccttgagt ttggccaaga agatcctaaa cgacattcca gctgtgcagg
      241 agctctgcgc acccaacact ggtttgagtt cctccactga ggagttttta tccagtgagt
      301 tccagatccc gacgttgccg ctgctcaaat ctgaggattt aacactggag gagcgtgtgc
      361 agcggatgct catcggtctg gagcttcatc acagagtcct gctgaagctg ctggagccct
      421 gccgcccaga gaacctactg aacgacctcg cagagctgcg cacgctgctc ctcctccagg
      481 tgccctctgc tggtcctcag gtgttgccgc acacacacac tctctcggct ccactcagcc
      541 agttcaagct gcaggtgcgt gtgcgcgcga cactccggca gctgcgcagc ttcatgcagg
      601 acgtgttccg cagcctgcgc tgcatcagtg tgtctcaatg ctaactggat ccacaggacg
      661 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      721 ggggcggagg ccaaaacggt cgga
//
