LOCUS       sequence-122548-       17237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic fragment of Drosophila aubergine was cloned from w1 
            background and inserted into pattB plasmid to expression Aubergine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17237)
  TITLE     pattB-aubergine-WT-gf
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pattB-aubergine-WT-gf
FEATURES             Location/Qualifiers
     source          1..17237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2408..2477
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             complement(8067..8084)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(12396..12429)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(12436..16572)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     CDS             complement(join(13204..13818,13889..14020,14241..14556,
                     14618..15272,15347..15620,16022..16093))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     16545..16563
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(16596..16615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(16597..16615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(16622..16639)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16622..16638)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16631..16653)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(16779..17234)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16916..16937)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     17128..17147
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggctagaact agtgtcgaca
     2221 tgcccgccgt gaccgtcgag aacccgctga cgctgccccg cgtatccgca cccgccgacg
     2281 ccgtcgcacg tcccgtgctc accgtgacca ccgcgcccag cggtttcgag ggcgagggct
     2341 tcccggtgcg ccgcgcgttc gccgggatca actaccgcca cctcgacccg ttcatcatga
     2401 tggaccagat gggtgaggtg gagtacgcgc ccggggagcc caagggcacg ccctggcacc
     2461 cgcaccgcgg cttcgagacc gtgacctaca tcgtcgacat ctagaggtac cctcgagccg
     2521 cggccgctcc cttaggagcc tcagcatttg gagacagtgg tggctgaact gctggctgac
     2581 cgaatcctgt attctcacct ggactgcaag gaaccgcggg accaaattgt tgcttcacat
     2641 ttagcgatgt tgtggagaaa aagagccgca caccacactg agtaacggcc acaagatgta
     2701 gtttgtcggc atcgtcggcg ctcagagggc agatggcttt cacactttta aaaatggacg
     2761 ggtccacagt cctaaagttt aaataatggt tattaattaa ccatactaca gttaaattct
     2821 ttgcgaaact agcacttacg tgatcagact aactgcttga ttcgtaatat cgttctgggt
     2881 aatccgacct agtcttcgag ctgtcgtgta actcgtactg atgtcccaag cctcaatgac
     2941 acctttttcc gtaaggacat agagaagctt gcgactgtta tcgatctcaa tgtgttcgat
     3001 tggatccact tcctgcaaac aaatcgcgct aaatagttac ggacattttt tgaagtaaag
     3061 acaatagtta ccgaaaagac cttcaaaaaa ctgggaacca tataggagac cagtccttgg
     3121 gagagattta ttttcttgca tcgcttgcca aaccaactgg actcggcctg atagtaaatt
     3181 tcatagaggc agccatcgcg accgcccaaa aagatgcggc cgtcatccgt tcctttgatg
     3241 acgctgatgg acacgttgtc ggtaccaatc acaaaaactg gccggttcat cagctgcatt
     3301 tcgttgtacg agctctcacc gaatgtcacg cccaggacaa tgacctcaat gggcgtagtg
     3361 agaaccagga ggtacttgac gtcctgcaca aagacgccgg gttttggttt caccaggcca
     3421 acgcttacga tcagatgact gaggccatcg tagtacgcca catccctcgt ctgattgaac
     3481 gtccagatgt agatttccga atcgatagtc agccaggcac gtccgatttc cggaaagagg
     3541 cccatggtgc agtggcattt tatgtctaca acaggtagga tcgaagattg gaacggatag
     3601 atctatgggc tatgaagccc aggatacgta cgcttaaagt gctccagaat ctcgttggga
     3661 atagccgact tggtcaccgt tcgcatttgt tgtaggttat gtgttctatc cgatttcaaa
     3721 aacgacaggc tctggtaatc gtagtcatta agtccgctca tcgtggcccg cccatgctgg
     3781 ctaacgccgg tcagctacaa agaacgtatc attaaaggag gagaagctga taaacatccg
     3841 tatccaatac atacttccag cagacccggc ttatttctct ccagattgta atgccattcc
     3901 agcatactgg tcgccgtcgt gaaaaagtcc agctgtgctt gcggaagagt catcgtgctg
     3961 aaaactgtga tgaggcgggc taactcgggt gcaatgctaa cttaaaatgc cgtcagacca
     4021 ctgttttggg atgaaactac aaactaatct gaagatttgg tcgactgatg ctcaatgtac
     4081 ccacgctccc gccttctgct aagtgttacc agatatcctc acattctcgg tattgttttg
     4141 gtatatttcg attgaatatt aatggatttc acaatttcag gcgccttagc ggggtattat
     4201 gaaatcgtct gtcacaagaa acatttgcaa acgtgcatga aattaatatt ttaataataa
     4261 tttcttttac tgctaatata tattatatga taaggacgcg tatattaata gacaataatg
     4321 gttttagtag gcatattcag tgggcttaaa tactttgaaa aaacgttgat aattttatat
     4381 aaacagtaac tgtgctaata caagtataat aaaaaaagca cttcccatgc cggaaacttt
     4441 tgagaatata aaaatattag aatgtgaaga gtataactca gttgagcgca acaggtgtgt
     4501 tattctctaa aatcggtaaa acctcaaact actgaatgaa ttcagcattt cgaagctttg
     4561 ccaatggatt ttataattgt gattaaaatc cactttccgc tttcccaaat agacttgatt
     4621 taagccttct tttcaaaaaa aaaaaaaaag actactgtaa ctcaacacag aaactctagc
     4681 gcttaacagc acaatgcgta acgttaacag caccccaacg gcacatggcg ctcaaaattt
     4741 agcgcatttt gtgcgcctca cacagccaca ttaaatttcc cttcttttct tttccccgtt
     4801 tccggtgagt agtgtgttcc gcgttatatt tttattgaaa ttttaaccca aaatatctct
     4861 aaaaccaccc cttgcagcga ggagtccatc cagaatttgt gtcatacaaa cgagggacta
     4921 acggagcgta agttttgtgt gaaaaagcgc cccaaaacat cgaatcgagt gaaacggcaa
     4981 ttggcgtgtg gcactgaaga gccgccgttt ttccatttcc gctgggatgg atgatccata
     5041 aacaaattgg aaaacaatag gcagcgcacg ttttgttagc ccgatgtgaa ctctgcgatt
     5101 cgatgtttgg aatgctctgt gacaagcact tgtagccttg ataaactaat aattaatgtt
     5161 tttgccgaca gtgtcatcaa gatgcgtacc attaattcca accagtgcgt gaagatccct
     5221 aaggatatca aggcctcggt gaaggcccgt gtggtgacca tcaccggcac ccgcggcacc
     5281 ctgaagcgca gcttcaagca cttggctctg gacatgtaca tgcccgacaa gcgcaccctg
     5341 aaggtggaga agtggttcgg aaccaagaag gagttggccg ccgtccgcac cgtctgcagt
     5401 cacatcgaga acatgatcaa gggtgcgtac agctagggta aacacaacag gaacactttc
     5461 acagccaggc ctttcatttg ccaatcacac gagaatttaa taataatatc tgggaagacg
     5521 atttactaaa ctccattcat agatatcaat tttctttaca aattacctaa aaatgctata
     5581 tagtagttag taaatatttt cagtgtgtgc tggcaaatta gattcggcat taggttccta
     5641 aagacatttg cattcaaagt accacacaat taattgcaat ttctctctcg ccaggagtca
     5701 cgtttggatt ccagtacaag atgcgtgctg tgtacgccca tttccccatc aactgtgtca
     5761 cctccgagaa caacacggtc attgagatcc gtaacttctt gggtgagaag tacatccgtc
     5821 gtgtggagat ggctcctggc gtcaccgtgg tcaactccac tgcccagaag gacgaactta
     5881 tcgtggaggg aaacgatatt gagtctgtct ccggatctgc cgccctcatc cagcagtcca
     5941 cgaccgtcaa gaacaaggat atccgtaagt tccttgacgg tctgtacgtg tccgagaaga
     6001 cgaccgttgt gaagctagag tcttagactt ttggaatgtg tttcaaccta aaatgttaat
     6061 aaaaaaaaac caacggccga aactttcaat aagaaattta gtgtctttaa acttttgtaa
     6121 tataatgtat tcagatttta tcacaatgct ccactaaaaa agtgatgatt ggttgaaaat
     6181 tcgttggtaa gattgtgtta tttatcttat ttatttattt gcaaggtttc ataagttcat
     6241 gataacttga gattgaaagc actacaaaag gacctttagg aagagatccc gcttaaggca
     6301 gaggtagtcc tctgctggtt ccctctggca cacatatgga aactgaacct tgcactctat
     6361 atcgtgatac ccaaaggctc cattgtaatt ggcgaatcca acacaatcct cttcggggga
     6421 agcgttgttc ggttccaggg gtacccagtt taggtacggc acagtttcgc cgttcctggc
     6481 gagaagaaag gtgcgccggt tacccaagca attgatgcca gcccaaacgg agtgatgcca
     6541 gctttgggtg aaggatagcc ccatactctt taggaacgtg gttgtgagga gtttgtcctc
     6601 ttgattgtcg agcaccatta gttcggcacc aagttttcga cagcttcgat cggcaacatg
     6661 ccagttggcc tgaaataata tttttgcgtt taaataaaat ccttgtacaa cgtattgtac
     6721 gcacctcctg caaagatacg tggtagcact tgtttccgac tcgactaaac ggcaacggac
     6781 acgtctgtgc attggcacat tcgagaatca gagctataag aaggaaaccg aaagaccctt
     6841 tgatgcacat gctcatgttt tagggccaac tgatatgttt atatgaaagc tagctacttt
     6901 taaacgctcg gcagagaact atattttatt gcaagcagtc gggagataat atttattaac
     6961 gttatcttaa gcaaattagg gcaatcagtg cgaaaatggc aagcatgtac aacgactgcg
     7021 aatttatcat tatcatttat tacaaaaata ttcaaagcct gttaatttac tatgccatct
     7081 ggacgatggc acatacgccc cttttacatt taaattgcac aaaggaatat aaaaaaagaa
     7141 cttataattt taatatgaaa gatcttataa aaatttggaa ttatggaatt acacctgtca
     7201 aaattccaat tgttccactg agatatctcg ttatatagac cttgttttga attcaacgat
     7261 gccttttcaa taagtaccac cttcaaacat ttaaacgtta aaataaataa tttcatatta
     7321 attttatttt tgttttatga cttgatatat agataatata tacaataaat agtaagtata
     7381 agctacatct ttgcaaatgt gtccggagat ttacaattaa tcgctttaag cgcgaccgat
     7441 aactgaagga ataactcaca tccctgggcg tctaacaact acccggtaat ttctgttatc
     7501 tcttattcac tgaacggcat ttgtgacgag tagatcgtaa gaaagtgcaa ctgtaatttg
     7561 tgtaattatg aatttaccac caaaccctgt aattgctcgt ggacgtggtc gaggaagaaa
     7621 gcccaataat gtagaggcca atcgcggctt cgctccttcg ctggtaggca tttatgtatt
     7681 ttcatgtttt atttttatcc aacttatcca aaattagctt ggccttgtct tttaatgtgc
     7741 atcccatttc aatttctatg taagatcagc atgcgtatca aatgtatgta tgcataatgt
     7801 tattgtgtgt acatcgtttg tacattacat tacatttttg taatacgtta aggtcgaata
     7861 gcatgtttgt acatcatttt gccatgagtt gttataacag tttattaata tgcatgtgac
     7921 aataaaaaca aatacatgct aattaaataa cactttgtta taaaataagt gtaattagat
     7981 taaataatca attttttaac caattcattc gcagggtcag aagtcggacc cttcccactc
     8041 ggaaggaaat caagcatccg gaggcaatgg tggtggtggt gatgctcaag tgggtccctc
     8101 gatagagaaa tccagtttga gcgcggtgca aatgcacaag tccgagggtt agtagtttta
     8161 tttggttttg ctcaaaaaaa tttgtttttt tttttttcga acccagcttc tgtgagctgt
     8221 tgttattctc atttgttttc aatgcgttcg taattaatta gtaatttaat tcaggcgatc
     8281 cgcgtggctc cgtgcgcggc cgtaggctaa taacggattt ggtgtattca cgtcctccag
     8341 ggatgaccag caagaaagga gtggttggca ctcatattac cgtgcaggca aactatttta
     8401 aggtattaaa gcgtccaaac tggaccatct accagtaccg cgtcgatttt acgcctgatg
     8461 tggaggctac acgactgcga cggtctttct tgtatgaaca taaagggatc ctgggcggct
     8521 acatctttga cggaaccaat atgttttgca tcaatcagtt caaagctgtt caagatagcc
     8581 cctatgtttt ggaactggtt acgaagagtc gtgctggcga aaacattgaa attaagatca
     8641 aggctgttgg atctgtgcaa tctacggatg ccgagcagtt tcaggtcctt aatctcatac
     8701 tgcgcagggc catggagggc ttagacttga agctggtctc gcgctactac tacgatcctc
     8761 aagctaaggt ttgccctctg tactcacatt tatcatcact ctcactttgg taatttaaaa
     8821 atttagatta atttggagaa tttccgcatg caattatggc ctggctatca gacttcgatt
     8881 cgccagcacg aaaatgatat attactttgt tccgagatat gccacaaggt tatgcgaact
     8941 gagaccttgt acaatatttt gtccgatgct attcgcgaca gtgacgatta tcaaagtaca
     9001 tttaagcgtg cagtaatggg tgagtttgat tatggttctc tcaagttctt attgtccaat
     9061 ttaaatactt cttaaaaggt atggtaatac taacggatta taataacaaa acctatcgca
     9121 ttgacgatgt cgactttcaa tcgacgccat tgtgcaaatt taaaaccaat gacggtgaga
     9181 tttcgtacgt ggattactat aagaaggtaa tattcgaacc aaaagcgtaa ttcattttcc
     9241 ataagtaaaa tctttttcag cgatacaaca taatcattcg cgacttgaag caacctctgg
     9301 tcatgtctcg tccgacagac aaaaatattc gtggtggaaa tgaccaggcc ataatgatca
     9361 ttcccgagct ggcacgggct acgggaatga cggacgctat gcgcgctgac tttaggtgtg
     9421 ttttgatgga atcagttgtg atccaaagga tgctattaaa tcaccgtttt tataattctc
     9481 aggacgttga gggccatgag tgaacatacc aggctgaatc cagatcgtcg catcgaacgc
     9541 cttcgcatgt tcaacaagcg cttaaagtcg tgtaaacaga gtgtagagac ccttaagtcc
     9601 tggaacatcg agctggactc cgccttggtg gagattccag cacgcgtgtt gccaccggaa
     9661 aaaatattat tcggcaacca aaaaatattc gtatgcgacg ctcgcgccga ttggaccaat
     9721 gagtttcgca cctgttcgat gtttaaaaac gtgcatatca ataggtggta tgtgatcaca
     9781 ccgagtcgaa atctgcgcga gacccaggaa tttgtgcaga tgtgcatccg cacagccagc
     9841 agcatgaaga tgaacatctg caatccaata tagtaagtat tactaactat gataaatgct
     9901 taatataatt tgacattcct tgtagtgagg aaattccgga tgaccgcaac ggcacttact
     9961 cccaagcgat cgacaacgcc gcagctaacg atccacagat cgtgatggtt gtcatgagat
    10021 ctccaaatga agagaagtaa ttagcactag attaacccca actaagccag tgctaactac
    10081 tttcccccat attgcaacag atatagctgc attaaaaaac gcacgtgcgt ggacagaccg
    10141 gtgccatcgc aggtggtgac actaaaagtt atcgcgcctc gacagcaaaa accaactggg
    10201 ctgatgtcga tcgccacaaa ggtggttatt caaatgaacg ccaaattgat gggagctccc
    10261 tggcaggtag tgatccccct ccacggtctg atgactgttg gtttcgatgt ctgccattca
    10321 ccgaagaata agaacaagtc atatggggct ttcgttgcaa ccatggacca gaaggagtct
    10381 ttccgctact tctccaccgt aaacgaacac attaagggcc aagagttgtc cgagcagatg
    10441 tcggtgaaca tggcgtgcgc cctgaggtcg tatcaggagc aacaccgttc tttgccagag
    10501 cgcattctct tctttcgcga cggtgttggc gatggtcagc tctaccaggt ggtaaacagc
    10561 gaggtgaaca ccctaaagga caggctcgac gaaatttaca aatcagctgg caaacaggag
    10621 ggctgtcgca tgacatttat aattgtatcc aagcgcatta attctcgcta ctttactggg
    10681 catcgcaacc cagttccggg cactgtagtc gatgacgtta ttaccttgcc agagcgctac
    10741 gacttcttcc tagtgtccca ggctgttcgg ataggaactg tgtcgcctac cagctacaat
    10801 gttatttctg acaacatggg actaaacgcc gataagctgc agatgctctc ctataagatg
    10861 acccatatgt actacaatta cagcggaacc atacgagtcc ccgctgtctg ccattacgcc
    10921 cacaaattgg ctttcctcgt agccgaatcc attaatcgtg cgccttcagc aggactgcag
    10981 aatcaattgt actttttgta aaactatgac ttgcattaaa tgcgttaaca attttacgat
    11041 ttgatatatg tatgtagata tgtacatcat ttgaatttaa taaaggtatt gcacatttat
    11101 tgtctaccaa tctgaaataa tcgttgtttc ttttaaatat gtaaatgttt ttgtctataa
    11161 agttccgttg ctggaaaatg catatattcg tatgaaatga gcttagtaaa taagttaaca
    11221 caatttacaa ttaacacaat atacaattta ataattcact gcaacttttt ccaatgcaca
    11281 attacatata aatttcaatt gttgccgaac acgtgcaatg ctgttttaat cctaatattt
    11341 cattttaaat caaagttgtt ttgttgctga ccaaacggag tgttgatcag ttgaaaagat
    11401 ccatttctaa taggaatttc cacattcact ccatcaaagt ctaaaagaaa aattattctg
    11461 tgtgagtgct tgcaggagta atcgaacttt aaagaggttc ggctcacctg gtcaacccct
    11521 taaatgcaga tgctaggact cgatttcctc ttttacagta tttggcgtcg ctggtgttgt
    11581 ttcttcatcg ttcgagtcct aaattaaaaa ttgaacagtt aacggaaatg tatacttaga
    11641 ttgaaaaggt aattttaaat agcgaaaaaa agcgaatata aaatacaatc ggatttccga
    11701 agttctcaga tcaataattg tcaactacta tacaaaacga ccaatttaaa taataattta
    11761 gacgcaaatt tcggccagtg cataggatca caggcatgca tgacaaaaaa tggtaagcaa
    11821 agggaaacta tataggttta aaacagtcgt aataaaatta agtgtgcacc taagatttta
    11881 caatggatac atatgtatat cactttatag tcccatgtat aacttatgtt aaagtattat
    11941 aaagtatatt aaatacaaaa taatatcgta ttgatttttc tctatttgac aattatatac
    12001 tacgtttaca cactaaatta aaatacattc ttcaatcaag aaattatact tatagcaagt
    12061 ttatcaactt gtttatttac atgtaagtac tgtccaatac aaaaatagaa gaaatttacg
    12121 aaaacaactc gttttaatca gattaattaa ttttgtactt tttaacatgt acttaccctt
    12181 cgatataaag ttaattatgg ttttaagaaa tatctattat ataatatact aaatttgatg
    12241 gattaagttg aatctagaca gcgattcttt aaatgcttaa tgtgaaaaca gattgttttg
    12301 atatttgtta ttttatagta ttagcaattg gtagggcaac cttatgcagc ggccgcagat
    12361 ctgttaacga attcccaatt cccggatccg ctagcataac ttcgtatagc atacattata
    12421 cgaagttatg ctagcctagt tccagtgaaa tccaagcatt ttctaaatta aatgtattct
    12481 tattattata gttgttattt ttgatatata taaacaacac tattatgccc accatttttt
    12541 tgagatgcat ctacacaagg aacaaacact ggatgtcact ttcagttcaa attgtaacgc
    12601 taatcactcc gaacaggtca caaaaaatta ccttaaaaag tcataatatt aaattagaat
    12661 aaatatagct gtgagggaaa tatatacaaa tatattggag caaataaatt gtacatacaa
    12721 atatttatta ctaatttcta ttgagacgaa atgaaccact cggaaccatt tgagcgaacc
    12781 gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg
    12841 cggagagcgg gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa
    12901 ttatgaagag ggcgcgcgat ttgaaaagta tgtatataaa aaatatatcc cggtgtttta
    12961 tgtagcgata aacgagtttt tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa
    13021 gacaaatcca aagtctactt gtggggatgt tcgaagggga aatacttgta ttctataggt
    13081 catatcttgt ttttattggc acaaatataa ttacattagc tttttgaggg ggcaataaac
    13141 agtaaacacg atggtaataa tggtaaaaaa aaaaaacaag cagttatttc ggatatatgt
    13201 cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca cccggaagct
    13261 cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg agaagttaag
    13321 cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat
    13381 ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg cgtaacggaa
    13441 ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt tcaagaagaa
    13501 gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg ccatcgaggt
    13561 cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg ccaccagagt
    13621 gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga tcatcggata
    13681 ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt
    13741 gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca taaaaactgg
    13801 cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta aattattaat
    13861 atgcaaatgt attctaaaca agacttacat ttatcgtggc aaagacgttt tgaaaggtca
    13921 tgttggtcag gaagaggaag atggctccgt tgatattcat cacgcccact tgcgtgagtt
    13981 gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta aaatgttact
    14041 cgcatctcat taatattcat atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt
    14101 ttctgcaaaa ctataaacta tacatctcat tgaaaaaaac taagaagggt gtggaatcag
    14161 gcaattctaa ctaaaatcta gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg
    14221 tttccactgg aaccactcac cgttgtctga ataagtcgca cttttacgag gagtggttcc
    14281 ttgagcaccg acagccagga tcgccacagg accgcccgga actgcatgaa ccaggtggcc
    14341 ttgtaggtgt acccattctc cggctgctcc agtggcttct ccaaattttt ggtggccaac
    14401 aactgctcca tatcccgggc tactttgcta atagcaaaat tgtcgcatat cttggcgatc
    14461 cgatcacggg actcgatctc ccgtccgggc acaacggcca acacctgtac gtaaaagtcc
    14521 gccggattgt agttggtagg acactgggca cccacgctgg ataggagttg agatgtaatg
    14581 taatgctaga tacccttaat aaacacatcg aactcactag gaaaagaagt cgacggcttc
    14641 gctgggagtg cccaagaaag ctaccctgcc ctcggccatc agaaggatct tgtcaaagag
    14701 ctcaaacagc tcggaagacg gctgatgaat ggtcaggatg acggtcttgc ccttctgcga
    14761 cagcttcttc agcacctgga cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg
    14821 ctcatcgcag atcagaagcg gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt
    14881 cctttctccg ccggacagac ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca
    14941 tttgctgagc gaaagctcct ggatcacctg atccacgcgg gccactcgct gccgataggt
    15001 cagatgtcgt ggcatccgca ccatggcctg gaaaatcagg tgttccctgg ccgttaggga
    15061 gccgataaag aggtcatcct gctggacata ggcgcacctg gcctgcatct ccttggcgtc
    15121 cacaggttgg ccattgagca gtcgcatccc ggatggcgat acttggatgc cctgcggcga
    15181 tcgaaaggca agggcattca gcagggtcgt ctttccggca ccggaactgc ccatcacggc
    15241 caaaagttcg cccggatagg ccacgccgca aactgagttt caaattggta attggaccct
    15301 ttattaagat ttcacacaga tcagccgact gcgaatagaa actcaccgtt cttgagcaaa
    15361 tgtttcctgg gcgccggtat gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc
    15421 agctgccgcc atccggagcc cggctgattg accgccccaa agatgtccat attgtgccag
    15481 gcataggtga ggttctcggc tagttggccg ctccctgaac cggagtcctc cggcggactg
    15541 ggtggccgga gcgtgccgta gtttttggcc tgcccgaagc cctggttaat gcagctctgc
    15601 gaagccgctc cgctgtcacc ctgcaatgat aggggatctc aaatatcaac tacaagcgtt
    15661 atgctcatct aaccccgaac aaaaagtacc ccgaagtatc ctacgaagta ggtttatact
    15721 tttatttatt ttttgtgcat ctaggatcag cttaaaatat ctggttgtta tattttttgt
    15781 aaaaaagaat atagtcgaaa atgaatgcct ttagatgtct tgatcatgat atgatctcaa
    15841 aaattgtctt atatagcgag aacagctacc agaataatct gtttcgtgtc actatttgtt
    15901 tgtgcaattg cggtttggga tttttgtggg tcgcagttct cacgccgcag acaatttgat
    15961 gttgcaatcg cagttcctat agatcaagtg aacttaagat gtatgcacat gtactactca
    16021 cattgttcag atgctcggca gatgggtgtt tgctgcctcc gcgaattaat agctcctgat
    16081 cctcttggcc cattgccggg atttttcaca ctttcccctg cttacccacc caaaaccaat
    16141 caccacccca atcactcaaa aaacaaacaa aaataagaag cgagaggagt tttggcacag
    16201 cactttgtgt ttaattgatg gcgtaaaccg cttggagctt cgtcacgaaa ccgctgacaa
    16261 aatgcaactg aaggcggaca ttgacgctac gtaacgctac aaacggtggc gaaagagata
    16321 gcggacgcag cggcgaaaga gacggcgata tttctgtgga cagagaagga ggcaaacagc
    16381 gctgactttg agtggaatgt cattttgagt gagaggtaat cgaaagaacc tggtacatca
    16441 aatacccttg gatcgaagta aatttaaaac tgatcagata agttcaatga tatccagtgc
    16501 agtaaaaaaa aaaaatgttt tttttatcta ctttccgcaa aaatgggttt tattaactta
    16561 catacatact aggggcccgg tacgtaccca attcgcccta tagtgagtcg tattacaatt
    16621 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    16681 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    16741 gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt
    16801 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
    16861 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
    16921 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
    16981 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
    17041 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
    17101 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
    17161 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    17221 gctacagggc gcgtcag
//
