LOCUS       sequence-122569-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Roseburia intestinalis Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX395 Roseburia intestinalis Cas9
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        84..193
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             248..274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tggatcttaa tacgactcac tataggggaa
       61 ttgtgagcgg ataacaattc cgggaccttt aattcaaccc aacacaatat attatagtta
      121 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      181 tttatttaca atcaacaagt ttgtacaaaa aagcaggcta aggagatata ccaccggtgc
      241 caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga
      301 agcgtccatg agagagaacg ggagtgacga aaggcggagg aatatggacg aaaaaatgga
      361 ttacaggatt gggctggaca tcggcatcgc atcagtgggg tgggccgtcc tgcagaacaa
      421 ttcagacgat gagcctgtga ggattgtcga cctgggagtg cgcattttcg ataccgcaga
      481 gatcccaaag acaggcgaaa gcctggcagg accccggaga gcagctcgaa ccacaaggcg
      541 ccgactgcgg agaaggaaac accgcctgga ccgaatcaag tggctgttcg agaaccaggg
      601 gctgatcaat attgacgatt ttctgaagag atacaacatg gccggactgc cagatgtgta
      661 ccagctgcgg tatgaggctc tggacagaaa actgaccgat gaggaactgg cccaggtgct
      721 gctgcacatc gctaagcatc ggggcttcag aagcaccagg aaagccgaga cagcagccaa
      781 ggaaaacggg gcagtgctga aggccacaga cgagaatcag aaacgaatgc aggaaaaggg
      841 atacaggaca gtgggcgaga tgatctacct ggacgaggcc ttccggactg gctgctcttg
      901 gagtgagaaa gggtacatcc tgaccccc
//
