LOCUS       sequence-122570-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Roseburia intestinalis Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX395 Roseburia intestinalis Cas9
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     71..88
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ccaggagagg cactggggag gggtcacagg gatgccaccc gaccactttg tacaagaaag
       61 ctgggttacg atttaggtga cactataggc ggccgcaagc ttgagctcgg taccgaattc
      121 ttagctaaag cccatccgct tttcggatcg gaccacgctg acatgtccca gcacatcgat
      181 ctgacacttc tccagcacgt ccagagactg aatgcccagt ccgcggaatt tatacctgga
      241 atcgtgggca atgccctgga tatttgcagc gctgatgtcg gctccaatat agtatgcgta
      301 ggtttccttg atcccctcca tgcctccgtt caccatctta atagggatat cctttttaga
      361 cttgatatgg atcaggtcgc gtgaatacag actaaacagg aaatccttgt cctccatcac
      421 tttccactcg ccgtatggct tgtgagctgt actggcccta ttaggcagca cctttttgac
      481 cacgtcagcg gtgtagacgg gcacaaaata gtacttgccg ttgactcgga acacatcgat
      541 gcgcaccatc ccgccattct cggcaatccc gttgccagag tcgacgaaca ctcccatggt
      601 ctgctttttc tggatcttca ccttcctgac cacagggccc tcagttccat cggccttggg
      661 tttatgaaag tcctgagcga aggccttttt ggcatcattg ccatacagca gcagctgctt
      721 tttcagtgct tcgtacagca gcagatcgga ctgggggtta tagtatccgt cgatctcccc
      781 atccttgtcc agtttcaggt cggtcagtgc ggtcttagtc agcacgatgc cctcatcttt
      841 aaagtgtctt ggggaccgaa ttgtgtcagc atgggctgct ccggtgacct tgtgatttgg
      901 cattctgctc acgaagatag gccgcaggtt ttcgtagatg tactcgggat aatccagctc
      961 cagagccaca tcgctatgag tgtc
//
