LOCUS       sequence-122571-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sphaerochaeta globus str. Buddy Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX391 Sphaerochaeta globus str. Buddy Cas9
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        84..193
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             248..274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tggatcttaa tacgactcac tataggggaa
       61 ttgtgagcgg ataacaattc cgggaccttt aattcaaccc aacacaatat attatagtta
      121 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      181 tttatttaca atcaacaagt ttgtacaaaa aagcaggcta aggagatata ccaccggtgc
      241 caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga
      301 agcgtccatg agcaagaaag tcagtagacg ctatgaagaa caggcacagg agatttgtca
      361 gaggctgggg agtagacctt attccatcgg gctggacctg ggagtgggat ctatcggagt
      421 ggcagtcgcc gcttacgacc caattaagaa acagccctcc gatctggtgt tcgtcagctc
      481 caggattttt atcccttcta ccggcgcagc cgagcggaga cagaagagag gacagaggaa
      541 cagcctgcgc caccgagcaa atcgcctgaa attcctgtgg aagctgctgg ctgaacgaaa
      601 cctgatgctg tcatatagcg agcaggacgt gccagatcct gcacggctga gatttgagga
      661 cgctgtggtc cgagcaaacc catacgagct gcggctgaag ggcctgaatg aacagctgac
      721 cctgagcgag ctggggtatg cactgtacca catcgccaat cataggggat ctagttcagt
      781 gcgcacattc ctggatgagg aaaagagctc cgacgataag aaactggagg aacagcaggc
      841 tatgacagaa cagctggcaa aagagaaggg aatttccact ttcatcgaag tgctgacagc
      901 ctttaacact aatggcctga tcgggtacag gaactccgag tctgtgaaga gtaagggcgt
      961 gccagtcccc actcgcgaca tcatttcaaa tgagattgat gtgctgctgc ag
//
