LOCUS       sequence-122572-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sphaerochaeta globus str. Buddy Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX391 Sphaerochaeta globus str. Buddy Cas9
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     71..88
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ccaggagagg cactggggag gggtcacagg gatgccaccc gaccactttg tacaagaaag
       61 ctgggttacg atttaggtga cactataggc ggccgcaagc ttgagctcgg taccgaattc
      121 ttactctcca cacagagcga cttcatcctt ctcgattttg tccaccagca gagggctgac
      181 gtatctcacc agcccggcac tagacagctg gactttccgc acatcataat tcttgatggt
      241 agtcagggta aaactagtgt cctgtccctt tttggacttg gccaggttgc tgaagtagat
      301 ctgatatcca gaccccatct ctgtaaaggt gataatgatc acgaaggttc gcccaggttt
      361 agcattgggc aggcggacat gtgtggtctt ggccaggttg gactctgctt cagtcagatc
      421 gcttgccctc agctcgcaca cgtcgccctg gtacagtctc acgtacagag aatacccctg
      481 tttcatgtat gctggcttat aggaagggtt catagcctgg atttttctaa tgatctcgcc
      541 acacagcttt ccctgggcat cgtgataaaa gtccaggcac agattgcttc cagtgtcaaa
      601 tgcgaaccct ttcacctcgt ttgaaggttc cttgaccacg aaagtctttg tgcgcttgtt
      661 gttgctaatc aggtaatagt acccgcccac cagctccagg gccttttttg aaattgtttt
      721 ttcactcagc tggatgggct ttttgcctga ctcaatcagc ttcctattac tctcttccag
      781 gacagcggct gcctgcacca gactctcctt gtatttctgc agagcggctt cgttcttaga
      841 ctgcagctgc tcgatcttat ctttcatttc catgggatac cactttggct gtttgtcggt
      901 gatccggcct cgaatggcag atgcgacctc ttcataatcc ccgattttca cgccgatgtc
      961 cttaatcttt ttcttgacca cgaacaccag atcctctccc tgggtgtctg ctcccagaat
     1021 tgaatacaca gtatctttca gcagg
//
