LOCUS       sequence-122584-        2252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., 
            Nat. Chem. Biol., 2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAOUTA4
FEATURES             Location/Qualifiers
     source          1..2252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(95..199)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     267..285
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(289..332)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(556..573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(727..1315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(807..826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1486..2252,1..25,26..94))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2109..2128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
       61 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      121 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      181 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
      241 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
      301 aaaaatcctt agctttcgct aaggatgatt tctggaattc taaagatctt tgacagctag
      361 ctcagtccta ggtataatga attctcgcac atcttgttgt ctgattattg atttttggcg
      421 aaaccatttg atcatatgac aagatgtgta tgcctctacc tgcttcggcc gataaagccg
      481 acgataatac tcccaaagcc cgccgaaagg cgggcttttt tttggatcct tactcgagtc
      541 tagactgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      721 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2221 cactcgtgca cccaactgat cttcagcatc tt
//
