LOCUS       sequence-122588-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-CBPDHFR
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             30..52
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             66..143
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     complement(728..746)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aattggaatt cgctagctct agaaataatt ttgtttaact ttaagaagga gatatacata
       61 tgtctaagcg acgatggaaa aagaatttca tagccgtctc agcagccaac cgctttaaga
      121 aaatctcatc ctccggggca cttctgggat ccatcagtct gattgcggcg ctagcggtag
      181 atcgcgttat cggcatggaa aacgccatgc catggaacct gcctgccgat ctcgcctggt
      241 ttaaacgcaa caccttaaat aaacccgtga ttatggggcg ccatacctgg gaatcaatcg
      301 gtaggccttt gcccggccgc aaaaatatta tcctcagcag tcaacccggg accgatgatc
      361 gggttacctg ggttaaatcg gtcgacgaag ccatcgcggc gtgtggtgac gtaccagaaa
      421 tcatggtgat tggcggcgga cgcgtttatg aacagttctt gccaaaagcg caaaaactgt
      481 atctgacgca tatcgatgca gaagtggaag gcgacaccca ttttccggat tacgagccgg
      541 atgactggga atcggtattc agcgaattcc acgatgctga tgcgcagaac tcgcatagct
      601 attgtttcga aatcctcgag cgtcgtgccg gctaatgtac aagatccaga tctaagcttg
      661 gtaccacgcg tgcgcgctga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      721 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg gg
//
