LOCUS       sequence-122593-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-FLAGDHFR
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..45
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..82
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(664..682)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attcgctagc tctagaaata attttgttta actttaagaa ggagatatac atatgggaga
       61 ctacaaggac gatgatgaca agggatccat cagtctgatt gcggcgctag cggtagatcg
      121 cgttatcggc atggaaaacg ccatgccatg gaacctgcct gccgatctcg cctggtttaa
      181 acgcaacacc ttaaataaac ccgtgattat ggggcgccat acctgggaat caatcggtag
      241 gcctttgccc ggccgcaaaa atattatcct cagcagtcaa cccgggaccg atgatcgggt
      301 tacctgggtt aaatcggtcg acgaagccat cgcggcgtgt ggtgacgtac cagaaatcat
      361 ggtgattggc ggcggacgcg tttatgaaca gttcttgcca aaagcgcaaa aactgtatct
      421 gacgcatatc gatgcagaag tggaaggcga cacccatttt ccggattacg agccggatga
      481 ctgggaatcg gtattcagcg aattccacga tgctgatgcg cagaactcgc atagctattg
      541 tttcgaaatc ctcgagcgtc gtgccggcta atgtacaaga tccagatcta agcttggtac
      601 cacgcgtgcg cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      661 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggt
//
