LOCUS       sequence-122594-         572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-FLAGDHFR
FEATURES             Location/Qualifiers
     source          1..572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(487..510)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             524..546
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggcacga cgctcgagga tttcgaaaca atagctatgc gagttctgcg catcagcatc
       61 gtggaattcg ctgaataccg attcccagtc atccggctcg taatccggaa aatgggtgtc
      121 gccttccact tctgcatcga tatgcgtcag atacagtttt tgcgcttttg gcaagaactg
      181 ttcataaacg cgtccgccgc caatcaccat gatttctggt acgtcaccac acgccgcgat
      241 ggcttcgtcg accgatttaa cccaggtaac ccgatcatcg gtcccgggtt gactgctgag
      301 gataatattt ttgcggccgg gcaaaggcct accgattgat tcccaggtat ggcgccccat
      361 aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg agatcggcag gcaggttcca
      421 tggcatggcg ttttccatgc cgataacgcg atctaccgct agcgccgcaa tcagactgat
      481 ggatcccttg tcatcatcgt ccttgtagtc tcccatatgt atatctcctt cttaaagtta
      541 aacaaaatta tttctagagc tagcgaattc ca
//
