LOCUS       sequence-122601-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-CBPNDHFR
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             65..142
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             167..187
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attggaattc gctagctcta gaaataattt tgtttaactt taagaaggag atatacatat
       61 gtctaagcga cgatggaaaa agaatttcat agccgtctca gcagccaacc gctttaagaa
      121 aatctcatcc tccggggcac ttctgggatc tggtggtggt ggtggtgaaa acctgtactt
      181 ccagggatcc atcagtctga ttgcggcgct agcggtagat cgcgttatcg gcatggaaaa
      241 cgccatgcca tggaacctgc ctgccgatct cgcctggttt aaacgcaaca ccttaaataa
      301 acccgtgatt atggggcgcc atacctggga atcaatcggt aggcctttgc ccggccgcaa
      361 aaatattatc ctcagcagtc aacccgggac cgatgatcgg gttacctggg ttaaatcggt
      421 cgacgaagcc atcgcggcgt gtggtgacgt accagaaatc atggtgattg gcggcggacg
      481 cgtttatgaa cagttcttgc caaaagcgca aaaactgtat ctgacgcata tcgatgcaga
      541 agtggaaggc gacacccatt ttccggatta cgagccggat gactgggaat cggtattcag
      601 cgaattccac gatgctgatg cgcagaactc gcatagctat tgtttcgaaa tcctcgagcg
      661 tcgtgccggc taatgtacaa gatccagatc taagcttggt accacgcgtg cgcgctgatc
      721 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct g
//
