LOCUS       sequence-122602-         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-CBPNDHFR
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(345..365)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(390..467)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     RBS             481..503
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(570..589)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(571..589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atatgcgtca gatacagttt ttgcgctttt ggcaagaact gttcataaac gcgtccgccg
       61 ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcgtc gaccgattta
      121 acccaggtaa cccgatcatc ggtcccgggt tgactgctga ggataatatt tttgcggccg
      181 ggcaaaggcc taccgattga ttcccaggta tggcgcccca taatcacggg tttatttaag
      241 gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc atggcatggc gttttccatg
      301 ccgataacgc gatctaccgc tagcgccgca atcagactga tggatccctg gaagtacagg
      361 ttttcaccac caccaccacc agatcccaga agtgccccgg aggatgagat tttcttaaag
      421 cggttggctg ctgagacggc tatgaaattc tttttccatc gtcgcttaga catatgtata
      481 tctccttctt aaagttaaac aaaattattt ctagagctag cgaattccaa ttggagctct
      541 ccggagccgg agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
      601 tcgatgcatg gtgccaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
      661 gatt
//
