LOCUS       sequence-122604-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-STRNDHFR
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..38
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(617..635)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctggtggtgg tggtggtgaa aacctgtact tccagggatc catcagtctg attgcggcgc
       61 tagcggtaga tcgcgttatc ggcatggaaa acgccatgcc atggaacctg cctgccgatc
      121 tcgcctggtt taaacgcaac accttaaata aacccgtgat tatggggcgc catacctggg
      181 aatcaatcgg taggcctttg cccggccgca aaaatattat cctcagcagt caacccggga
      241 ccgatgatcg ggttacctgg gttaaatcgg tcgacgaagc catcgcggcg tgtggtgacg
      301 taccagaaat catggtgatt ggcggcggac gcgtttatga acagttcttg ccaaaagcgc
      361 aaaaactgta tctgacgcat atcgatgcag aagtggaagg cgacacccat tttccggatt
      421 acgagccgga tgactgggaa tcggtattca gcgaattcca cgatgctgat gcgcagaact
      481 cgcatagcta ttgtttcgaa atcctcgagc gtcgtgccgg ctaatgtaca agatccagat
      541 ctaagcttgg taccacgcgt gcgcgctgat ccggctgcta acaaagcccg aaaggaagct
      601 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      661 gtctt
//
