LOCUS       sequence-122609-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FKBP12 only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Varnai P, Thyagarajan B, Rohacs T, Balla T
  TITLE     Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate 
            levels influence multiple regulatory functions of the lipid in 
            intact living cells.
  JOURNAL   J Cell Biol. 2006 Nov 6;175(3):377-82.
  PUBMED    17088424
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-11-6"; volume: "175(3)"; pages: "377-82"
COMMENT     Sequence Label: mRFP-FKBP12
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..728
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             54..728
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(184..202)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     452..471
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     646..669
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggcct
       61 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
      121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      181 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      241 ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      301 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      421 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      481 gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga
      541 tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg
      601 ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct
      661 cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca
      721 ccggcgcctc cggactca
//
