LOCUS       sequence-122625-        6111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Yokosaki Y, Palmer EL, Prieto AL, Crossin KL, Bourdon MA, Pytela R, 
            Sheppard D
  TITLE     The integrin alpha 9 beta 1 mediates cell attachment to a non-RGD 
            site in the third fibronectin type III repeat of tenascin.
  JOURNAL   J Biol Chem. 1994 Oct 28. 269(43):26691-6.
  PUBMED    7523411
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1994 Oct 28. 269(43):26691-6."
COMMENT     Sequence Label: integrin alpha9 pBS
FEATURES             Location/Qualifiers
     source          1..6111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3874..3890)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3874..3890)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3926..3944)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3965..3981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3965..3981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3978..4000)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3989..4005
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4013..4019,4020..4037,4038..4043))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4058..4079
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4196..4213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4367..4955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4447..4466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5126..5917,5918..5986))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5749..5768
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5987..6091)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgatag cttagagcgg gcgctatggg gagccggacg
      721 ccagagtccc ctctccatgc cgtgcagctg cgctggggcc cccggcgccg acccccgcta
      781 ctgccgctgc tattgctgct cgtgccgccg ccaccgcggg tcgggggcta caacctggat
      841 ccgcagcgcc ccgtgcactt ccagggcccc gctgactcgt tcttcggcta cgcagttctg
      901 gagcatttcc acgacaacac gcgctgggtc cttgtgggcg caccaaaggc agattccaaa
      961 tacagccctt cagtgaagtc tcctggggct gtgtttaagt gccgtgttca caccaaccct
     1021 gaccggagat gcaccgaact ggacatggct cgagggaaga atcggggcac gtcctgcgga
     1081 aagacctgcc gggaagaccg cgatgatgag tggatggggg tgagcctggc ccgacagccc
     1141 aaggctgatg gccgtgtgtt ggcctgtgct catcgctgga agaacatcta ctatgaagcc
     1201 gaccacatcc taccccatgg cttctgctac atcatcccct ccaacctcca ggccaaaggc
     1261 aggacgctga tcccttgcta tgaagagtat aagaagaagt acggagagga acacggctcc
     1321 tgccaggctg ggatagcggg cttcttcacc gaggagctgg tggtgatggg tgctccaggg
     1381 tcattttatt gggctggaac catcaaagtg ctgaacctta cggacaacac ctatttaaaa
     1441 ctgaacgacg aagtgatcat gaacaggcgg tacacctacc tgggctacgc agtgaccgct
     1501 ggccacttct ctcacccgtc caccattgat gtggtaggag gtgccccaca ggacaaaggc
     1561 atcggcaagg tttatatttt cagagctgac cgaagatcag gcaccttaat taagatcttt
     1621 caagcatcag gtaaaaagat gggctcttac ttcggctcct ccttgtgcgc agttgacctg
     1681 aatggggacg gcctctctga cctgctggtg ggggccccca tgttttctga gatcagggat
     1741 gagggacagg tcactgtcta catcaacaga ggaaatggag ccctcgagga gcagctggct
     1801 ctgactgggg atggtgccta caatgcccac tttggagaga gcattgccag cctggacgat
     1861 ctggacaatg atgggttccc agatgtggcc attggtgcac ccaaggagga tgacttcgca
     1921 ggggcggtct atatctatca tggtgatgcc ggtgggatag tccctcagta ctcaatgaaa
     1981 ctgtctgggc agaagataaa tccagtgctc cggatgtttg gtcagtccat atcgggaggc
     2041 attgatatgg atggaaatgg ctatcctgat gtcactgttg gagccttcat gtccgacagc
     2101 gtggttcttc tcagagcaag gcctgtcatt acggtggatg tctccatctt cctcccgggc
     2161 tccatcaaca tcacagcgcc tcagtgtcac gacggacagc agcctgtgaa ctgcctgaac
     2221 gtcaccacct gcttcagctt ccatggcaaa cacgttccag aagagattgg cctgaattat
     2281 gttctgatgg ctgacgtggc caaaaaggag aagggccaga tgcccagggt ctactttgtg
     2341 ctgctgggag agaccatggg tcaggtcaca gagaagctgc agctgactta catggaggag
     2401 acgtgtcgtc actatgtggc ccatgtgaag cggagggtgc aggacgtcat cagcccgatc
     2461 gtgtttgaag cagcctacag cctcagtgag catgtgactg gagaggagga gagggaactg
     2521 ccgcctctga caccagttct ccgctggaaa aagggacaaa agattgccca aaagaatcag
     2581 actgtttttg aaaggaattg ccgttcagag gactgtgccg cagacctgca gcttcagggt
     2641 aaactgctgc tctccagtat ggatgagaaa accctgtatc tagctttggg ggctgtgaag
     2701 aacatctccc taaacatctc tatctccaac ctcggagatg atgcctatga tgccaacgtg
     2761 tccttcaatg tttcccggga gctcttcttc atcaacatgt ggcagaagga ggagatgggc
     2821 atctcctgtg agctgctgga atcggacttc ctcaaatgca gcgtgggatt tcctttcatg
     2881 aggtcaaagt caaagtatga attcagcgtg atctttgata caagccacct gtctggggaa
     2941 gaggaagttc tcagcttcat tgttactgct cagagtggca acacggagcg ctctgaatcc
     3001 ctgcatgaca acaccctcgt gctgatggtg ccactgatgc acgaggtgga cacgtccatc
     3061 accggaatca tgtctccaac ctcctttgta tatggcgagt ccgtggacgc agccaacttc
     3121 attcagctgg atgacctgga gtgtcacttt cagcccatca atatcaccct tcaggtctac
     3181 aacactggcc caagcaccct tccagggtca tctgtcagca tctctttccc taatcgactc
     3241 tcatctggtg gtgcagagat gtttcatgtc caggaaatgg tggtgggcca agagaaggga
     3301 aactgctctt tccagaaaaa cccaactccc tgcatcatcc ctcaagaaca agaaaatatc
     3361 ttccacacaa tatttgcttt tttcacaaag tctggaagaa aagtcttgga ctgtgaaaaa
     3421 ccaggaattt cttgcctaac agcacactgt aactttagtg ctcttgctaa agaagaaagt
     3481 cgtactatag acatttacat gctgctgaac acagaaatac tgaaaaagga cagttcgtct
     3541 gtcatccagt tcatgtcccg cgccaaggtg aaggtggatc ctgccctaag ggtggtggaa
     3601 atagctcatg ggaacccaga agaggtgacg gtggtcttcg aggccctgca caatctggag
     3661 ccccgtggct acgtcgtggg gtggatcatc gccatcagtt tgttggtggg aatcctcatc
     3721 ttcctgctgc tggccgtgct gctctggaag atgggcttct ttcgccgaag gtacaaagaa
     3781 attatcgaag ctgagaagaa ccggaaagag aatgaagaca gttgggactg ggtccagaaa
     3841 aaccagtgag ctgccacacc agtcacatga cctgatccac tagttctaga gcggccgcac
     3901 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     3961 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4021 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4081 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4141 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4201 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4261 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4321 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4381 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4441 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4501 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4561 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4621 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4681 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4741 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4801 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4861 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4921 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4981 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5041 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5101 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5161 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5221 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5281 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5341 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5401 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5461 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5521 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5581 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5641 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5701 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5761 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5821 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5881 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5941 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6001 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6061 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg c
//
