LOCUS       sequence-122638-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRB conjugated plasma membrane targeting sequences 2 from 
            Lyn11(GCIKSKGKDSA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Varnai P, Thyagarajan B, Rohacs T, Balla T
  TITLE     Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate 
            levels influence multiple regulatory functions of the lipid in 
            intact living cells.
  JOURNAL   J Cell Biol. 2006 Nov 6;175(3):377-82.
  PUBMED    17088424
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-11-6"; volume: "175(3)"; pages: "377-82"
COMMENT     Sequence Label: PM2-FRB-CFP
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(138..851,852..854))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(183..204)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     532..551
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     791..812
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctgcataac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 ctagagtcgc ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      181 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      241 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      301 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      361 aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgatg
      421 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      481 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      541 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
      601 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccccag
      661 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
      721 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
      781 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
      841 tcgcccttgc tcacaagg
//
