LOCUS       sequence-122639-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRB conjugated plasma membrane targeting sequences 2 from 
            Lyn11(GCIKSKGKDSA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Varnai P, Thyagarajan B, Rohacs T, Balla T
  TITLE     Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate 
            levels influence multiple regulatory functions of the lipid in 
            intact living cells.
  JOURNAL   J Cell Biol. 2006 Nov 6;175(3):377-82.
  PUBMED    17088424
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-11-6"; volume: "175(3)"; pages: "377-82"
COMMENT     Sequence Label: PM2-FRB-CFP
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..128)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             143..421
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             428..454
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(470..472,473..931)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(512..533)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(773..792)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcacc atgggatgta taaaatcaaa
       61 agggaaagac agcgcgggag cagatcccac cagatctgcg aattcaggtg ctggtgctgg
      121 tgctggtgct atcctatcta gaatcctctg gcatgagatg tggcatgaag gcctggaaga
      181 ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc
      241 cttgcatgct atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc
      301 ctatggtcga gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa
      361 tgtcaaggac ctcacccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa
      421 gactagttat ccgtacgacg taccagacta cgcaccggtc gccaccctgg tgagcaaggg
      481 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      541 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      601 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      661 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      721 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      781 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      841 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      901 ctacatcagc cacaacgtct atatcaccgc cgacaagca
//
