LOCUS       sequence-122646-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of GC localized oxBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha 
            VV, Snapp EL
  TITLE     A palette of fluorescent proteins optimized for diverse cellular 
            environments.
  JOURNAL   Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
  PUBMED    26158227
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-9"; volume: "6:7670. doi"; pages: " 
            10.1038/ncomms8670"
COMMENT     Sequence Label: GalToxBFP
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      254..263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(260..262,263..265,266..979)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(305..326)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct cgccaccatg aggcttcggg agccgctcct
       61 gagcggcagc gccgcgatgc caggcgcgtc cctacagcgg gcctgccgcc tgctcgtggc
      121 cgtctgcgct ctgcaccttg gcgtcaccct cgtttactac ctggctggcc gcgacctgag
      181 ccgcctgccc caactggtcg gagtctccac accgctgcag tcgacggtac cgcgggcccg
      241 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      301 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg
      361 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atcagtacta ccggcaagct
      421 gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagg tgttcgcccg
      481 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      541 ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
      601 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcgtcgact tcaaggagga
      661 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca tctatatcat
      721 ggccgtcaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acgtggagga
      781 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      841 gctgctgccc gacagccact acctgagcac ccagtccgtg ctgagcaaag accccaacga
      901 gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca ctctcggcat
      961 ggacgagctg tacaagtaa
//
