LOCUS       sequence-122647-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of ER localized moxGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha 
            VV, Snapp EL
  TITLE     A palette of fluorescent proteins optimized for diverse cellular 
            environments.
  JOURNAL   Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
  PUBMED    26158227
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-9"; volume: "6:7670. doi"; pages: " 
            10.1038/ncomms8670"
COMMENT     Sequence Label: ERmoxGFP
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..93
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVST"
     CDS             join(103..105,106..816)
                     /codon_start=1
                     /product="monomeric GFP variant optimized for use in 
                     oxidizing environments such as the secretory pathway 
                     (Costantini et al., 2015)"
                     /label=moxGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQSFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(145..166)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atggacagca aaggttcgtc gcagaaaggg tcccgcctgc tcctgctgct ggtggtgtca
       61 aatctactct tgtgccaggg tgtggtctcc accggaccgg tcgtgtccaa gggcgaggag
      121 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      181 ttctccgtgc ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc
      241 atcagcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      301 ggcgtgcaga gcttctcccg ctaccccgac cacatgaagc gccacgactt cttcaagagc
      361 gccatgcccg aaggctacgt ccaggagcgc accatctcct tcaaggacga cggcacctac
      421 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      481 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac
      541 tcccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
      601 atccgccaca acgtggagga cggctccgtg cagctcgccg accactacca gcagaacacc
      661 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgtccac ccagtccaag
      721 ctgtccaaag accccaacga gaagcgcgat cacatggtcc ttctggaatt cgtgaccgcc
      781 gccgggatca ctcacggcat ggacgagctg tacaagaagg acgagctgta agcggccgcg
      841 a
//
