LOCUS       sequence-122652-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 N863A nickase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX429 SpCas9 N863A nickase
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             68..88
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac
       61 catggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa
      121 gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
      181 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
      241 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct
      301 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
      361 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
      421 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
      481 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
      541 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
      601 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
      661 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
      721 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa
      781 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
      841 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaa
//
