LOCUS       sequence-122653-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 N863A nickase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX429 SpCas9 N863A nickase
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..57)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gaattcttac tttttctttt ttgcctggcc ggcctttttc gtggccgccg gccttttgtc
       61 gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat
      121 cagggtggcg tccagcacct ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc
      181 aaagtacttg aaggcggcag gggctcccag attggtcagg gtaaacaggt ggatgatatt
      241 ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg taggcggaca gcactttgtc
      301 cagattagcg tcggccagga tcactctctt ggagaactcg ctgatctgct cgatgatctc
      361 gtccaggtag tgcttgtgct gttccacaaa cagctgtttc tgctcattat cctcggggga
      421 gcccttcagc ttctcatagt ggctggccag gtacaggaag ttcacatatt tggagggcag
      481 ggccagttcg tttcccttct gcagttcgcc ggcagaggcc agcattctct tccggccgtt
      541 ttccagctcg aacagggagt acttaggcag cttgatgatc aggtcctttt tcacttcttt
      601 gtagcccttg gcttccagaa agtcgatggg attcttctcg aagctgcttc tttccatgat
      661 ggtgatcccc agcagctctt tcacactctt cagtttcttg gacttgccct tttccacttt
      721 ggccaccacc agcacagaat aggccacggt ggggctgtcg aagccgccgt acttcttagg
      781 gtcccagtcc ttctttctgg cgatcagctt atcgctgttc ctcttgggca ggatagactc
      841 tttgctgaag ccgcctgtct gcacctcggt ctttt
//
