LOCUS       sequence-122659-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pasteurella multocida Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX406 Pasteurella multocida Cas9
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        84..193
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             248..274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tggatcttaa tacgactcac tataggggaa
       61 ttgtgagcgg ataacaattc cgggaccttt aattcaaccc aacacaatat attatagtta
      121 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      181 tttatttaca atcaacaagt ttgtacaaaa aagcaggcta aggagatata ccaccggtgc
      241 caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga
      301 agcgtcccag actaccaacc tgagttacat cctgggactg gacctgggaa tcgcaagtgt
      361 cggctgggct gtcgtggaaa tcaatgaaaa cgaggaccct atcggcctga ttgatgtggg
      421 ggtccgaatc ttcgaacgag cagaggtgcc aaagaccgga gagtccctgg cactgtctcg
      481 gagactggcc agatccacaa ggcgcctgat tcgacggaga gctcacaggc tgctgctggc
      541 aaagagattt ctgaaaaggg agggaatcct gtctaccatt gacctggaaa agggcctgcc
      601 caaccaggcc tgggaactga gagtggctgg cctggagagg cgcctgagtg ctattgaatg
      661 gggggcagtc ctgctgcacc tgatcaaaca tcgcggatac ctgagcaagc gaaaaaacga
      721 gtcccagacc aacaataagg aactgggagc actgctgtcc ggagtggcac agaatcacca
      781 gctgctgcag tctgacgatt acagaacacc tgccgagctg gctctgaaga aattcgctaa
      841 ggaggaaggc cacatccgca accagcgagg ggcatatacc cataccttca acaggctgga
      901 cctgctggcc gagctgaatc tgctgttcgc tcagcagcat cagtttggca atccacactg
      961 caaagagcat atcc
//
