LOCUS       sequence-122667-        7896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SNX2-PRDM6 fusion gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Yao F, Kausalya JP, Sia YY, Teo AS, Lee WH, Ong AG, Zhang Z, Tan JH,
            Li G, Bertrand D, Liu X, Poh HM, Guan P, Zhu F, Pathiraja TN, 
            Ariyaratne PN, Rao J, Woo XY, Cai S, Mulawadi FH, Poh WT, Veeravalli
            L, Chan CS, Lim SS, Leong ST, Neo SC, Choi PS, Chew EG, Nagarajan N,
            Jacques PE, So JB, Ruan X, Yeoh KG, Tan P, Sung WK, Hunziker W, Ruan
            Y, Hillmer AM
  TITLE     Recurrent Fusion Genes in Gastric Cancer: CLDN18-ARHGAP26 Induces 
            Loss of Epithelial Integrity.
  JOURNAL   Cell Rep. 2015 Jul 14;12(2):272-85. doi: 
            10.1016/j.celrep.2015.06.020. Epub 2015 Jul 2.
  PUBMED    26146084
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.020"; journalName: "Cell Rep"; date: 
            "2015-07-14- 14"; volume: "12"; issue: "2"; pages: "272-85"
COMMENT     Sequence Label: pMXs-Puro-SNX2-PRDM6
FEATURES             Location/Qualifiers
     source          1..7896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1920..1929
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1924..1933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4173..4199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4236..4256)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4241..4570
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4421..4556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4580..5179
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4580..4599)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5076..5096
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5401..5870
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6000..6017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6171..6759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6251..6270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6923..7714,7715..7783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7546..7565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7784..7888)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaagccg
     1921 ccaccatggc ggccgagagg gaacctcctc cgctggggga cgggaagccc accgactttg
     1981 aggatctgga ggacggagag gacctgttca ccagcactgt ctccacccta gagtcaagtc
     2041 catcatctcc agaaccagct agtcttcctg cagaagatat tagtgcaaac tccaatggcc
     2101 caaaacccac agaagttgta ttagatgatg acagagaaga tctttttgca gaagccacag
     2161 aagaagtttc tttggacagc cctgaaaggg aacctatcct atcctcggaa ccttctcctg
     2221 cagtcacacc tgtcactcct actacactca ttgctcctag aattgaatca aagagtatgt
     2281 ctgctcccgt gatctttgat agatccaggg aagagattga agaagaagca aatggagaca
     2341 tttttgacat agaaattggt gtatcagatc cagaaaaagt tggtgatggc atgaatgcct
     2401 atatggcata tagagtaaca acaaagacat ctctttccat gttcagtaag agtgaatttt
     2461 cagtgaaaag aagattcagc gactttcttg gtttgcacag caaattagca agcaaatatt
     2521 tacatgttgg ttatattgtg ccaccagctc cagaaaagag tatagtaggg atgaccaagg
     2581 tcaaagtggg taaagaagac tcatcatcca ctgagtttgt agaaaaacgg agagcagctc
     2641 ttgaaaggta tcttcaaaga acagtaaaac atccaacttt actacaggat cctgatttaa
     2701 ggcagttctt ggaaagttca gagctgccta gagcagttaa tacacaggct ctgagtggag
     2761 caggaatatt gaggatggtg aacaaggctg ccgacgctgt caacaaaatg acaatcaaga
     2821 tgaatgaatc ggatgcatgg tttgaagaaa agcagcagca atttgagaat ctggatcagc
     2881 aacttaggaa acttcatgtc agtgttgaag ccttggtctg tcatagaaaa gaactttcag
     2941 ccaacacagc tgcctttgct aaaagtgctg ccatgttagg taattctgag gatcatactg
     3001 ctttatctag agctttgtct cagcttgcag aggttgagga gaagatagac cagttacatc
     3061 aagaacaagc ttttgctgac ttttatatgt tttcagaact acttagtgac tacattcgtc
     3121 ttattgctgc agtgaaaggt gtgtttgacc atcgaatgaa gtgctggcag aaatgggaag
     3181 atgctcaaat tactttgctc aaaaaacgtg aagctgaagc aaaaatgatg gttgctaaca
     3241 aaccagataa aatacagcaa gctaaaaatg aaataagaga gatatatgac caggatggga
     3301 cactacagca ctttattgat ggtggggaac ctagtaagtc gagctggatg aggtatatcc
     3361 gatgtgcaag gcactgcgga gaacagaatc taacagtagt tcagtacagg tcgaatatat
     3421 tctaccgagc ctgtatagat atccctaggg gcaccgagct tctggtgtgg tacaatgaca
     3481 gctatacgtc tttctttggg atccccttac aatgcattgc ccaggatgaa aacttaaatg
     3541 tcccttcaac ggtaatggaa gccatgtgca gacaagacgc cctgcagccc ttcaacaaaa
     3601 gcagcaaact cgcccctacc acccagcagc gctccgttgt tttcccccag actccgtgca
     3661 gcaggaactt ctctcttctg gataagtctg ggcccattga atcaggattt aatcaaatca
     3721 acgtgaaaaa ccagcgagtc ctggcaagcc caacttccac aagccagctc cactcggagt
     3781 tcagtgactg gcatctttgg aaatgtgggc agtgctttaa gactttcacc cagcggatcc
     3841 tcttacagat gcacgtgtgc acgcagaacc ccgacagacc ctaccaatgc ggccactgct
     3901 cccagtcctt ttcccagcct tcagaactga ggaaccacgt ggtcactcac tctagtgacc
     3961 ggcctttcaa gtgcggctac tgtggtcgtg cctttgccgg ggccaccacc ctcaacaacc
     4021 acatccgaac ccacactgga gaaaagccct tcaagtgcga gaggtgtgag aggagcttca
     4081 cgcaggccac ccagctgagc cgacaccagc ggatgcccaa tgagtgcaag ccaataactg
     4141 agagcccaga atcaatcgaa gtggatctcg agtacccata cgatgttcca gattacgctt
     4201 gatgagcggc cgccagcaca gtggtcgaca gctgtggaat gtgtgtcagt tagggtgtgg
     4261 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4321 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4381 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4441 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4501 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4561 cttttgcaaa aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4621 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4681 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4741 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4801 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4861 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4921 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4981 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5041 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5101 cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac
     5161 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     5221 acgacccatc gtcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     5281 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     5341 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     5401 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     5461 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     5521 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     5581 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     5641 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     5701 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     5761 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     5821 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
     5881 gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat
     5941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6841 tcaaaaagga tcttcaccta gatccttttg cggccggccg caaatcaatc taaagtatat
     6901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7861 agaaaaataa acaaataggg gttccgcgca catttc
//
