LOCUS       sequence-122670-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor empty vector with floxed puromycin resistance gene expression 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: PL552
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    88..121
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        182..681
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(217..240)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     614..633
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(721..740)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacgaa
       61 ttccgatcat attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta
      121 ttaggtctga agaggagttt acgtccagcc aagcttagga tctcgacctc gaaattctac
      181 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
      241 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
      301 cgccaaccga ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
      361 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
      421 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
      481 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
      541 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
      601 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
      661 tcctcatctc cgggcctttc gacctgcatc catctagatc tcgagcagct gaagcttacc
      721 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
      781 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
      841 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
      901 atcggcaagg tgtggg
//
