LOCUS       sequence-122674-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and
            Neo selection gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: AAVS1-Neo-CAG-Flpe-ERT2
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(104..123)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
       61 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
      121 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
      181 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
      241 catcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
      301 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
      361 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
      421 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
      481 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
      541 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
      601 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
      661 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
      721 catagcc
//
