LOCUS       sequence-122675-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: Kan-MB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_2_Kan-MB1
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(251..270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
       61 tttctaatca gaattggtta attggttgta acactggcag agggctcgat cggtgtgaaa
      121 agtcagtatc cagtcgtgta gttcttatta cctgtcccgt tgctggcgtt tttccatagg
      181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      721 tgcaagcagc agattacgcg cagaa
//
