LOCUS       sequence-122678-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expressing Cas9 and Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: Cas9-2A-Cre
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcggccgcca ccatggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc
       61 ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc
      121 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg
      181 gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag
      241 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct
      301 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac
      361 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat
      421 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc
      481 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca
      541 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc
      601 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg
      661 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc
      721 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc
      781 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac
      841 aatctgctgg
//
