LOCUS       sequence-122679-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expressing Cas9 and Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: Cas9-2A-Cre
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..93)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agttggggtg ggcgaagaac tccagcatga gatccccgcg ctgcagttac ttgtacacta
       61 tcacagatct tcttcagaaa taagtttttg ttccaccggt ccatcgccat cttccagcag
      121 gcgcaccatt gcccctgttt cactatccag gttacggata tagttcatga caatatttac
      181 attggtccag ccaccagctt gcatgatctc cggtattgaa actccagcgc gggccatatc
      241 tcgcgcggct ccgacacggg cactgtgtcc agaccaggcc aggtatctct gaccagagtc
      301 atccttagcg ccgtaaatca atcgatgagt tgcttcaaaa atcccttcca gggcgcgagt
      361 tgatagctgg ctggtggcag atggcgcggc aacaccattt tttctgaccc ggcaaaacag
      421 gtagttattc ggatcatcag ctacaccaga gacggaaatc catcgctcga ccagtttagt
      481 tacccccagg ctaagtgcct tctctacacc tgcggtgcta accagcgttt tcgttctgcc
      541 aatatggatt aacattctcc caccgtcagt acgtgagata tctttaaccc tgatcctggc
      601 aatttcggct atacgtaaca gggtgttata agcaatcccc agaaatgcca gattacgtat
      661 atcctggcag cgatcgctat tttccatgag tgaacgaacc tggtcgaaat cagtgcgttc
      721 gaacgctaga gcctgttttg cacgttcacc ggcatcaacg ttttcttttc ggatccgccg
      781 cataaccagt gaaacagcat tgctgtcact tggtcgtggc agcccggacc gacgatgaag
      841 catgtttagc tggcccaaat gttgctggat agtttttact gccagaccgc gcgcctgaag
      901 atata
//
