LOCUS       sequence-122680-         469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and
            blasticidin selection gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: AAVS1-Blasticidin-CAG-Flpe-ERT2
FEATURES             Location/Qualifiers
     source          1..469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aattctcatt aatttcgggt atatttgagt ggaatgagtt cttcaatcgt agttttgact
       61 aacttgccat tcatttctat taacacaaaa caatctggtg catagtctga aatcaactcc
      121 ctacacatac cacaaggact taccactcga atacttctat ctacttcgtc agaataaggg
      181 tgtctaacag ctacaatcgt gtcaaaatcc ttttgtccat tcgaaactgc actaccaatc
      241 gcaatggctt ctgcacaaac agttactcgt cctatatacg cttcaatatg tactgccgaa
      301 atgatttctc ctgttttcgt acgaattgcc gctcccacat gatgtttatt atcctcataa
      361 agcattgtaa tcttctctgt cgctacttct actaattcta gatcttgttg agaaatgtta
      421 aatgttttca taccggtcct agggccggga ttctcctcca cgtcaccgc
//
