LOCUS       sequence-122681-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and
            blasticidin selection gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, 
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001.
  PUBMED    26145478
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 
            10.1016/j.stem.2015.06.001."
COMMENT     Sequence Label: AAVS1-Blasticidin-CAG-Flpe-ERT2
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcac gcgtctatca
       61 agctgtggca gggaaaccct ctgcctcccc cgtgatgtaa tacttttgca aggaatgcga
      121 tgaagtagag cccgcagtgg ccaagtggct ttggtccgtc tcctccacgg atgcccctcc
      181 acggctagtg ggcgcatgta ggcggtgggc gtccgccgcc tccagcagca ggtcatagag
      241 gggcaccacg ttcttgcact tcatgctgta cagatgctcc atgcctttgt tactcatgtg
      301 cctgatgtgg gagaggatga ggaggagctg ggccagccgc tggtgctgct gctgcagggt
      361 caggcctgcc ttggccatca ggtggatcaa agtgtctgtg atcttgtcca ggactcggtg
      421 gatatggtcc ttctcttcca gagacttcag ggtgctggac agaaatgtgt acactccaga
      481 attaagcaaa ataatagatt tgaggcacac aaactcctct ccctgcagat tcatcatgcg
      541 gaaccgagat gatgtagcca gcagcatgtc gaagatctcc accatgccct ctacacattt
      601 tccctggttc ctgtccaaga gcaagttagg agcaaacagt agcttcactg ggtgctccat
      661 ggagcgccag acgagaccaa tcatcaggat ctctagccag gcacattcta gaaggtggac
      721 ctgatcatgg agggtcaaat ccacaaagcc tggcaccctc ttcgcccagt tgatcatgtg
      781 aac
//
