LOCUS       sequence-122690-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Treponema denticola Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX380 Treponema denticola Cas9
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        84..193
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             248..274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tggatcttaa tacgactcac tataggggaa
       61 ttgtgagcgg ataacaattc cgggaccttt aattcaaccc aacacaatat attatagtta
      121 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      181 tttatttaca atcaacaagt ttgtacaaaa aagcaggcta aggagatata ccaccggtgc
      241 caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga
      301 agcgtccatg aaaaaagaaa tcaaagacta cttcctgggg ctggatgtgg ggactgggag
      361 cgtggggtgg gctgtgaccg atactgacta caaactgctg aaggctaacc gaaaagacct
      421 gtggggcatg agatgcttcg agacagccga aactgctgag gtgcggagac tgcacagggg
      481 agccaggcgc cgaatcgagc ggagaaagaa acgcattaag ctgctgcagg agctgttctc
      541 tcaggaaatc gccaaaaccg atgagggctt ctttcagaga atgaaggaaa gcccctttta
      601 cgctgaggac aaaacaatcc tgcaggaaaa cactctgttc aatgacaagg attttgctga
      661 taagacttac cacaaagcat atcctaccat taatcatctg atcaaggctt ggattgagaa
      721 caaggtgaaa ccagaccccc gactgctgta cctggcatgt cacaacatca ttaagaaaag
      781 gggacatttc ctgtttgaag gcgacttcga ttcagagaat cagtttgata ccagcatcca
      841 ggcactgttc gagtatctgc gcgaggacat ggaggtggac atcgatgccg acagccagaa
      901 ggtcaaagag attctgaagg atagctccct gaagaactct gaaaaacaga gtcggctgaa
      961 taagatcctg gggctgaagc cttccgacaa acagaagaaa gccatcacaa acctgatttc
     1021 tggaaacaag atcaatttcg ccgatctgta cgacaatcca gatctgaagg acgctgagaa
     1081 aa
//
