LOCUS       sequence-122696-        6445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derivative of pSiM24 plant expression vector without the M24 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Sahoo DK, Dey N, Maiti IB
  TITLE     pSiM24 is a novel versatile gene expression vector for transient 
            assays as well as stable expression of foreign genes in plants.
  JOURNAL   PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
            eCollection 2014.
  PUBMED    24897541
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-4"; volume: "9(6):e98988. doi"; pages: " 
            10.1371/journal.pone.0098988. eCollection 2014"
COMMENT     Sequence Label: pSi
FEATURES             Location/Qualifiers
     source          1..6445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    67..91
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      283..924
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(2004..2183)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2418..2442
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2557..3145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2637..2656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3316..4107,4108..4176))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3939..3958
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4177..4281)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4322..4932
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4409..4940
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ggcgcgcggt acccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
       61 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
      121 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccactaga ccagtacctt
      181 ggaggcctgt gcgcatccca gggtgggtcc cgtatacccc ggggaattca agcttggatc
      241 cctcgaggtt aacacgcgtg tcgacgagct cctgcagtct agagctttcg ttcgtatcat
      301 cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca caccagaatc
      361 ctactgagtt cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc
      421 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
      481 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
      541 ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt
      601 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
      661 cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt cagacctaga
      721 aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca tttatgaact
      781 ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt ataattatag
      841 ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt tatgacttgc
      901 caattgattg acaacatgca tcaatcgata ctagtctagt aacatagatg acaccgcgag
      961 cgataattta tcctagtttg cgagctatat tttgttttct atcgcgtatt aaatgtataa
     1021 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
     1081 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
     1141 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgattcagaa gaactcatcg
     1201 aggagcctgt agaaagcaat cctttgggaa tctggagcag caattccgta aagcacaagg
     1261 aatctatcag cccactctcc accaagttct tcagcaatat ccctagtagc gagagcaata
     1321 tcctgatacc tatcagcaac tccaagcctt ccgcaatcaa tgaatccaga aaaccttccg
     1381 ttctccacca taatgtttgg aaggcaagca tcaccatgag taacaacaag atcctcacca
     1441 tctggcattc tagccttgag cctagcaaaa agttcagctg gagccaatcc ttgatgttcc
     1501 tcatcaagat catcctgatc cacaagtcca gcttccatcc tagttctagc cctttcaatt
     1561 ctgtgcttag cttggtgatc aaatgggcaa gtagcaggat caagagtgtg aagccttctc
     1621 atagcatcag ccataatgga aaccttctca gctggagcaa ggtgtgaaga aagcaaatct
     1681 tgtcctggaa cctctcccaa aagaagccaa tctcttccag cctcagtaac aacatcaaga
     1741 acagcagcgc atggaacacc agtagtagca agccaagaaa gtctagcagc ctcatcttga
     1801 agctcattaa gagcaccaga aagatcagtc ttaacgaaaa gaactggcct tccctgagca
     1861 gaaagcctaa aaacagcagc atcagagcat ccaatagtct gttgagccca atcgtatcca
     1921 aaaagcctct caacccaagc agcaggactt ccagcatgaa gtccatcttg ctcaattccc
     1981 atttgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
     2041 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
     2101 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctcag
     2161 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
     2221 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcagat ctggtgaccc
     2281 ctatgtaagg ggccggccat ttaaattcgc gattaattaa cctgtggttg gcatgcacat
     2341 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
     2401 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
     2461 tgaaggcggg aaacgacaat ctgatatcgc gcgcggtacc tgagcaaaag gccagcaaaa
     2521 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2581 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2641 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2701 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2761 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2821 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2881 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2941 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3001 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3061 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3121 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3181 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3241 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3301 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3361 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3421 cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga
     3481 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3541 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3601 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3661 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3721 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3781 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3841 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3901 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3961 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4021 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4081 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4141 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4261 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacgggccc cgctcaccgg
     4321 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
     4381 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa
     4441 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg
     4501 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc
     4561 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag
     4621 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg
     4681 ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac
     4741 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt
     4801 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt
     4861 taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc
     4921 tcgaaccctc ccggcccgct gtcgactacc tagatgtggc gcaacgatgc cggcgacaag
     4981 caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc
     5041 aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac
     5101 gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg
     5161 gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc
     5221 ggggcaatcc cgcaaggagg gtgaatgaat cgcaccttcg atcgcaaggc gtatcgccag
     5281 gaactgatcg acgccggctt ctcggccgaa gatgccgaaa cgatcgcctc gcgcaccgtc
     5341 atgcgtgccc cgcgcgaaac cttccagtcg gtcggctcga tggtccagca ggccaccgcc
     5401 aagatcgaac gcgattcggt ccagctggcc ccgccggccc ttccggcccc gtcggccgcc
     5461 gtcgaacgct cgcgccggct ggaacaggaa gccgccggcc tggccaagtc gatgaccatc
     5521 gacacccgcg gcaccatgac caccaagaag cgcaagaccg ccggcgagga tctggccaag
     5581 caggtcagcg aagccaagca ggccgccctg ctgaagcata ccaagcagca gatcaaggaa
     5641 atgcagctgt cgctgttcga catcgccccg tggccggata ccatgcgggc catgccgaat
     5701 gataccgccc gctcggccct gttcaccacc cgcaataaga agatcccgcg cgaagccctc
     5761 cagaataagg tcatcttcca cgtcaacaag gatgtcaaga tcacctatac cggcgtcgaa
     5821 ctgcgggccg atgatgatga actggtctgg cagcaggtcc tggaatatgc caagcgcacc
     5881 ccgatcggcg aaccgatcac cttcaccttc tatgaactgt gccaggatct gggctggtcg
     5941 atcaatggcc gctattatac caaggccgaa gaatgcctgt cgcgcctcca ggcgaccgcg
     6001 atgggcttca cctcggatcg cgtcggccat ctggaatcgg tcagcctgct gcaccgcttc
     6061 cgcgtcctgg atcgcggcaa gaagacctcg cgctgccagg tcctgatcga cgaagaaatc
     6121 gtcgtcctgt tcgccggcga tcattatacc aagttcatct gggagaagta tcgcaagctg
     6181 tcgccgaccg cccgccgcat gttcgattat ttctcgtcgc accgcgaacc gtatccgctg
     6241 aagctggaaa ccttccgcct gatgtgcggc tcggattcga cccgcgtcaa gaagtggcgc
     6301 gaacaggtcg gcgaagcctg cgaagaactg cgcggctcgg gcctggtcga acatgcctgg
     6361 gtcaatgatg atctggtcca ttgcaagcgc tagggccttg tggggtcagt tccggctggg
     6421 ggttcagcca gcgctttacg atatc
//
