LOCUS       sequence-122709-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
  TITLE     The tumor suppressor CYLD regulates entry into mitosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
  PUBMED    17495026
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 May 22. 104(21):8869-74."
COMMENT     Sequence Label: GFP-CYLD-STOP
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(14..42)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     637..657
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    719..743
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
       61 attttctgtc agtggagagg gtgaaggtga tgctacatac ggaaagctta cccttaaatt
      121 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta
      181 tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact ttttcaagag
      241 tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaacta
      301 caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa
      361 aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt acaactataa
      421 ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa
      481 aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc aacaaaatac
      541 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgc
      601 cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc
      661 tgctgggatt acacatggca tggatgagct ctacaaaagc ggttccggac cggatcaaac
      721 aagtttgtac aaaaaagttg gcgcgcccac catgagttca ggcttatgga gccaagaaaa
      781 agtcacttca ccctactggg aagagcggat tttttacttg cttcttcaag aatgcagcgt
      841 tacagacaaa caaacacaaa agctccttaa agtaccgaag ggaagtatag gacagtatat
      901 tcaagatcgt tctgtggggc attcaaggat tcct
//
