LOCUS       sequence-122727-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Dantas G, Kuhlman B, Callender D, Wong M, Baker D
  TITLE     A large scale test of computational protein design: folding and 
            stability of nine completely redesigned globular proteins.
  JOURNAL   J Mol Biol. 2003 Sep 12. 332(2):449-60.
  PUBMED    12948494
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2003 Sep 12. 332(2):449-60."
COMMENT     Sequence Label: PL2
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..70
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             290..307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(360..378)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      374..421
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(545..564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     755..776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actttaagaa ggagatatac atatgaaaga aaccgctgct gctaaattcg aacgccagca
       61 catggacagc ccagatctgg gtacctccgg cgacactacc gtgcgtgtga ttttcatctt
      121 tgccgatggt aaaacgacca caatcgaatt tacaggcagt gaggaagcag cgaaaaaaca
      181 agcccaggaa tatgcacaaa gcttacgcga taactacggc gattattcta tcgattacca
      241 aaatggcggc gaacttatta aaattgtatt ctctggttca ggcctcgagc accaccacca
      301 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      361 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      421 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      481 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      601 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      661 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      721 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      781 acactcaacc c
//
