LOCUS       sequence-122728-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Dantas G, Kuhlman B, Callender D, Wong M, Baker D
  TITLE     A large scale test of computational protein design: folding and 
            stability of nine completely redesigned globular proteins.
  JOURNAL   J Mol Biol. 2003 Sep 12. 332(2):449-60.
  PUBMED    12948494
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2003 Sep 12. 332(2):449-60."
COMMENT     Sequence Label: PL2
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(258..302)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             313..335
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    350..374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(374..393)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(375..393)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(481..500)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        706..783
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(803..822)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgaggcctga accagagaat
       61 acaattttaa taagttcgcc gccattttgg taatcgatag aataatcgcc gtagttatcg
      121 cgtaagcttt gtgcatattc ctgggcttgt tttttcgctg cttcctcact gcctgtaaat
      181 tcgattgtgg tcgttttacc atcggcaaag atgaaaatca cacgcacggt agtgtcgccg
      241 gaggtaccca gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct
      301 ttcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      361 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tcgatctcga
      421 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      481 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
      541 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
      601 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
      661 gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg
      721 cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
      781 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
      841 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggg
//
