LOCUS       sequence-122729-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SPL9 expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Rubio-Somoza I, Zhou CM, Confraria A, Martinho C, von Born P, 
            Baena-Gonzalez E, Wang JW, Weigel D
  TITLE     Temporal control of leaf complexity by miRNA-regulated licensing of 
            protein complexes.
  JOURNAL   Curr Biol. 2014 Nov 17;24(22):2714-9. doi: 
            10.1016/j.cub.2014.09.058. Epub 2014 Nov 6.
  PUBMED    25448000
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.09.058"; journalName: "Curr Biol"; date: 
            "2014-11-17- 17"; volume: "24"; issue: "22"; pages: "2714-9"
COMMENT     Sequence Label: pIR196
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(62..103)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgcggccct ctaggatcag cgggtttaaa ctcaatggtg atggtgatga tgaccggtac
       61 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaag ggccctctag
      121 atcgaaccac tttgtacaag aaagctgggt cgaattcgcc ctttcagaga gaccagttgg
      181 tatggtgaga agaagagtca taagcccttg tgttctcatc ttccatgtac tgtctcctac
      241 tttgatgatg gtgatcagat aactcgaact cacccattgc cgtgccacta cttatctgac
      301 aattatctgg ctcggtgtat cgacctaaat tcaaaacagg agacatatca ttatcattgt
      361 ccttgaatac ccaaggcggg ttcagatact gatgctggct aggtgcaggt ggtggttgag
      421 ccattgtaac cgtcatcggg ccaaaacctg aagaagctcg ccatgtattg ttgttgttgt
      481 tgttgttgtt gttgttgttg ttgttgttgt catgtggttg atgtggattg cttaacaagc
      541 ttaaggcgca gtttgagtcg ccaattccct tgtagctctc tagtttagtg tccataatct
      601 ctggagatga gaagcttgtc cctcctccac caactgaacc ttgactaaat acattcattg
      661 gattgatctg ccatgacggt gacgacactg gccgcctcat cactcttgta tccaatgttc
      721 ttgaacttgg ccatcctatc tcttggttcc caagaaagct tccattcatt ccagcatcac
      781 cattttcgta aagcgaaggt gcgatcctcc cgtaacgaga agctaacaca gagagagacg
      841 caggctgtgg cttccttcgt cgctcattat gaccagcgag tctcctgcgg caactccttt
      901 tctctaggtc aaatt
//
