LOCUS       sequence-122748-        5126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Castoreno AB, Wang Y, Stockinger W, Jarzylo LA, Du H, Pagnon JC, 
            Shieh EC, Nohturfft A
  TITLE     Transcriptional regulation of phagocytosis-induced membrane 
            biogenesis by sterol regulatory element binding proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34.
  PUBMED    16141315
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13129-34."
COMMENT     Sequence Label: pLDLR-Luc (aka: pES7)
FEATURES             Location/Qualifiers
     source          1..5126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      465..474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             471..2123
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(537..555)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2164..2285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2175..2194)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2229..2248
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2481..2498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2652..3240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2732..2751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3411..4202,4203..4271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4034..4053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4272..4376)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4403..4858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4490..4509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4700..4721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4989..5037
                     /note="synthetic polyadenylation signal"
     misc_feature    join(5051..5126,1..16)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5091..5110
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gccagaacat ttctctatcg ataggtaccg agctcttacg cgtgctagcc caattcgccc
       61 ttggctgttg ttatccttct gtggacaaca acagcaaaat gttaacagtt aaacatcgag
      121 aaatttcagg aggatctttc agaagatgcg tttccaattt tgagggggcg tcagctcttc
      181 accggagacc caaatacaac aaatcaagtc gcctgccctg gcgacacttt cgaaggactg
      241 gagtgggaat cagagcttca cgggttaaaa agccgatgtc acatcggccg ttcgaaactc
      301 ctcctcttgc agtgaggtga agacatttga aaatcacccc actgcaaact cctccccctg
      361 ctagaaacct cacattgaaa tgctgtaaat gacgtgggcc aagggcgaat tgggctcgag
      421 atctgcgatc taagtaagct tggcattccg gtactgttgg taaagccacc atggaagacg
      481 ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag
      541 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
      601 atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg
      661 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
      721 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
      781 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta
      841 ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc
      901 caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
      961 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag
     1021 agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc
     1081 tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag
     1141 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     1201 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     1261 taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa
     1321 gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat
     1381 acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg
     1441 gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg
     1501 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     1561 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     1621 ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca
     1681 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     1741 cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt
     1801 acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca
     1861 tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg
     1921 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     1981 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     2041 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     2101 agggcggaaa gatcgccgtg taattctaga gtcggggcgg ccggccgctt cgagcagaca
     2161 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2221 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2281 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2341 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
     2401 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     2461 gtcgccgcac cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3301 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4021 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4081 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4141 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4201 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4261 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4321 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4381 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4441 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4501 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4561 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4621 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4681 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4741 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4801 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc
     4861 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4921 ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga
     4981 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     5041 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     5101 aggctgtccc cagtgcaagt gcaggt
//
