LOCUS       sequence-122749-        5964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Castoreno AB, Wang Y, Stockinger W, Jarzylo LA, Du H, Pagnon JC, 
            Shieh EC, Nohturfft A
  TITLE     Transcriptional regulation of phagocytosis-induced membrane 
            biogenesis by sterol regulatory element binding proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13129-34.
  PUBMED    16141315
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 13. 102(37):13129-34."
COMMENT     Sequence Label: pLDLR-Luc mutSRE
FEATURES             Location/Qualifiers
     source          1..5964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    132..266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(691..711)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          841..906
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1147..2799)
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     2715..2733
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3266..3387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3577..4032)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3714..3735)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3926..3945
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4059..4163
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4164..4232,4233..5024)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4382..4401)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5195..5783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5684..5703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5937..5954
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
       61 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
      121 atcggtcgac ggatccagac atgataagat acattgatga gtttggacaa accacaacta
      181 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      241 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      301 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      361 ctgattatga tctctagtca aggcactata catcaaatat tccttattaa cccctttaca
      421 aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag acactctatg
      481 cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc tacttataaa
      541 ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt cctttgtggt
      601 gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca aaaaacttag
      661 caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg gaggagtaga
      721 atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg agcaaaacag
      781 gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat aggttggaat
      841 ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat
      901 atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa
      961 gtaaggttcc ttcacaaaga tccggcgaat ttctgccatt catccgctta ttatcactta
     1021 ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
     1081 cgccctgcca ctcatcgcag tattacaata gctaagaatt tcgtcatcgc tgaatacagt
     1141 tacattttac aatttggact ttccgccctt cttggccttt atgaggatct ctctgatttt
     1201 tcttgcgtcg agttttccgg taagaccttt cggtacttcg tccacaaaca caactcctcc
     1261 gcgcaacttt ttcgcggttg ttacttgact ggcgacgtaa tccacgatct ctttttccgt
     1321 catcgtcttt ccgtgctcca aaacaacaac ggcggcggga agttcaccgg cgtcatcgtc
     1381 gggaagacct gccacgcccg cgtcgaagat gttggggtgt tgtaacaata tcgattccaa
     1441 ttcagcgggg gccacctgat atcctttgta tttaattaaa gacttcaagc ggtcaactat
     1501 gaagaagtgt tcgtcttcgt cccagtaagc tatgtctcca gaatgtagcc atccatcctt
     1561 gtcaatcaag gcgttggtcg cttccggatt gtttacataa ccggacataa tcataggtcc
     1621 tctgacacat aattcgcctc tctgattaac gcccagcgtt ttcccggtat ccagatccac
     1681 aaccttcgct tcaaaaaatg gaacaacttt accgaccgcg cccggtttat catccccctc
     1741 gggtgtaatc agaatagctg atgtagtctc agtgagccca tatccttgtc gtatccctgg
     1801 aagatggaag cgttttgcaa ccgcttcccc gacttctttc gaaagaggtg cgcccccaga
     1861 agcaatttcg tgtaaattag ataaatcgta tttgtcaatc agagtgcttt tggcgaagaa
     1921 tgaaaatagg gttggtacta gcaacgcact ttgaattttg taatcctgaa gggatcgtaa
     1981 aaacagctct tcttcaaatc tatacattaa gacgactcga aatccacata tcaaatatcc
     2041 gagtgtagta aacattccaa aaccgtgatg gaatggaaca acacttaaaa tcgcagtatc
     2101 cggaatgatt tgattgccaa aaataggatc tctggcatgc gagaatctga cgcaggcagt
     2161 tctatgcgga agggccacac ccttaggtaa cccagtagat ccagaggaat tcattatcag
     2221 tgcaattgtt ttgtcacgat caaaggactc tggtacaaaa tcgtattcat taaaaccggg
     2281 aggtagatga gatgtgacga acgtgtacat cgactgaaat ccctggtaat ccgttttaga
     2341 atccatgata ataattttct ggattattgg taattttttt tgcacgttca aaattttttg
     2401 caaccccttt ttggaaacaa acactacggt aggctgcgaa atgttcatac tgttgagcaa
     2461 ttcacgttca ttataaatgt cgttcgcggg cgcaactgca actccgataa ataacgcgcc
     2521 caacaccggc ataaagaatt gaagagagtt ttcactgcat acgacgattc tgtgatttgt
     2581 attcagccca tatcgtttca tagcttctgc caaccgaacg gacatttcga agtattccgc
     2641 gtacgtgatg ttcacctcga tatgtgcatc tgtaaaagca attgttccag gaaccagggc
     2701 gtatctcttc atagccttat gcagttgctc tccagcggtt ccatcctcta gaggatagaa
     2761 tggcgccggg cctttcttta tgtttttggc gtcttccatt ttaccaacag taccggaatg
     2821 ccaagcttac ttagatctcg agcccaattc gcccttggcc cacgtcattt acagcatttc
     2881 aatgtgaggt ttctagcagg gggaggagtt tgcagtgggg ttattttcaa atgtcttcac
     2941 ctcactgcaa gaggaggagt ttcgaacggc cgatgtgaca tcggcttttt aacccgtgaa
     3001 gctctgattc ccactccagt ccttcgaaag tgtcgccagg gcaggcgact tgatttgttg
     3061 tatttgggtc tccggtgaag agctgacgcc ccctcaaaat tggaaacgca tcttctgaaa
     3121 gatcctcctg aaatttctcg atgtttaact gttaacattt tgctgttgtt gtccacagaa
     3181 ggataacaac agccaagggc gaattgggct agcacgcgta agagctcggt acctcccggg
     3241 ttatgttagc tcagttacag taccataaga tacattgatg agtttggaca aaccacaact
     3301 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3361 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3421 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctccacgt accttaatat
     3481 tacttactta tcatggtagc ttgggctggc gtaatagcga agaggcccgc accgatcgcc
     3541 cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt aatattttgt
     3601 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     3661 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     3721 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3781 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3841 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3901 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3961 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     4021 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4081 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4141 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4201 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4261 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4321 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4381 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4441 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4501 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4561 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4621 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4681 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4741 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4801 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4861 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4921 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4981 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5341 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5581 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5821 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5881 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5941 agtcagtgag cgaggaagcg gaag
//
