LOCUS       sequence-122754-        9718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for human KCC2 under CAG promoter. 
            Coexpression of TdTomato with IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Bortone D, Polleux F
  TITLE     KCC2 expression promotes the termination of cortical interneuron 
            migration in a voltage-sensitive calcium-dependent manner.
  JOURNAL   Neuron. 2009 Apr 16;62(1):53-71. doi: 10.1016/j.neuron.2009.01.034.
  PUBMED    19376067
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.01"; journalName: "Neuron"; date: 
            "2009-04-16- 16"; volume: "62"; issue: "1"; pages: "53-71"
COMMENT     Sequence Label: pCITF-KCC2
FEATURES             Location/Qualifiers
     source          1..9718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    958..1005
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1030..1049
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1237..2031)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1237..2031)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1348..1367)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1958..1977
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2032..2135)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2243..2546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2548..2825
                     /label=chicken beta-actin promoter
     intron          2826..3838
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3762..3784
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3846..3865
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7292..7318
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    7327..7900
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7350..7902
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7494..7511)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7721..7740
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7919..7928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7925..9352
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             9353..9376
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    9527..9608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9564..9583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      781 agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt
      841 cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat
      901 ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa
      961 caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc
     1021 cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact
     1081 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     1141 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     1201 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     1261 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     1321 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     1381 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     1441 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     1501 gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     1561 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     1621 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     1681 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     1741 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     1801 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt
     1861 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     1921 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     1981 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tactcttcct
     2041 ttttcaatat tattgaagca tttatcaggg tattgtctca tgagcggata catatttgaa
     2101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2161 gaacgaagca tctgtgcttc actagttatt aatagtaatc aattacgggg tcattagttc
     2221 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2281 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2341 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2401 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2461 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2521 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     2581 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     2641 cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
     2701 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     2761 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     2821 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
     2881 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     2941 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
     3001 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
     3061 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     3121 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     3181 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     3241 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     3301 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     3361 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     3421 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     3481 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     3541 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     3601 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     3661 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     3721 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     3781 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     3841 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgctcgagct
     3901 caagcttcga attcggctta tgcccaacaa cctgacggac tgcgaggacg gcgatggggg
     3961 agccaacccg ggtgatggca accccaagga aagcagtccc ttcatcaaca gcaccgacac
     4021 agagaaggga aaggagtatg atggcaagaa catggccttg tttgaggagg agatggacac
     4081 cagccctatg gtgtcctcct tgctcagtgg cctggccaac tacaccaacc tgccccaggg
     4141 aagtagggag catgaagagg cagaaaacaa tgagggtgga aaaaagaagc cggtgcaggc
     4201 cccacgcatg ggcaccttca tgggcgtgta cctgccgtgc ctgcagaaca tctttggcgt
     4261 catcctcttc ctgcggctca cctgggtggt gggcattgca ggcatcatgg agtccttctg
     4321 catggtgttc atctgctgct cctgtacgat gctcacggcc atctccatga gtgcaattgc
     4381 aacgaatggt gttgtgcctg ctggtggctc ctactacatg atttccaggt ctctgggccc
     4441 agagtttggg ggtgccgtgg gcctctgctt ctacctgggc actacctttg caggagccat
     4501 gtacatcctg ggcaccatcg aaatcctgct ggcttacctc ttcccagcca tggccatctt
     4561 caaggcagaa gatgccagtg gggaggcagc agccatgctg aacaacatgc gtgtttacgg
     4621 cacctgtgtg ctcacctgca tggccactgt ggtgtttgtg ggtgtcaagt atgtcaacaa
     4681 gtttgccctt gtcttcctgg gttgtgtcat cctctccatc ctggccatct atgctggggt
     4741 catcaagtct gccttcgacc cacccaactt cccgatctgc ctcctgggta accgcacgct
     4801 gtctcgccat ggctttgatg tctgtgccaa gctggcttgg gaaggaaatg agacggtgac
     4861 cacacggcta tggggccttt tctgctcctc tcgcttcctc aacgccacct gtgatgaata
     4921 cttcacccga aacaatgtca cagagatcca gggcatccct ggtgctgcca gtggcctcat
     4981 caaagagaac ctctggagct cctacctgac caagggcgtg attgtggaga ggagtgggat
     5041 gacctcggtg ggcctggccg atggcactcc tatcgacatg gaccaccctt atgtcttcag
     5101 tgatatgacc tcctacttca ccctgctggt tggcatctac ttcccctcag tcacagggat
     5161 catggctggt tctaaccgct ctggggacct gagggatgcc cagaagtcaa tccccactgg
     5221 caccatcctg gccatcgcca ccacctctgc tgtctacatc agctccgttg ttctgtttgg
     5281 ggcctgcatt gagggggtcg tcctgcggga caagtttggc gaagctgtga atggcaacct
     5341 cgtggtgggc actctggcct ggccatctcc atgggtaatt gtcatcggat ccttcttctc
     5401 cacctgtggg gctgggctgc agagcctcac gggggcccca cgcctgctgc aggccatctc
     5461 gagggatggc attgtgccct tcctgcaggt ctttggccat ggcaaggcca atggagagcc
     5521 gacctgggcc ctgctcctga ctgcctgcat ctgcgagatt ggcatcctca ttgcatccct
     5581 cgacgaggtg gcccccatcc tctctatgtt cttcctgatg tgctacatgt ttgtgaatct
     5641 ggcctgtgca gtgcagacgc tgctgaggac acccaactgg aggccacgct ttcgatatta
     5701 ccactggacc ctctccttcc tgggcatgag cctctgcctg gccctcatgt tcatctgctc
     5761 ctggtattat gcactggtag ccatgctcat tgctggactc atctacaagt acattgagta
     5821 ccgtggggca gagaaggagt ggggcgatgg gatacgaggt ctgtctctca gtgcggctcg
     5881 ctatgccctc ttacgcctgg aggaagggcc cccacacacc aagaactgga ggccacagct
     5941 gctggtgctg gtgcgtgtgg accaagacca gaatgtggtg cacccccagc tgctctcact
     6001 gacctcccag ctgaaggcag ggaagggcct gaccatcgtg ggctctgtcc ttgagggcac
     6061 ctttctggaa aatcatccac aggcccagcg ggcagaagag tctatcaggc gcctgatgga
     6121 ggcagagaag gtgaagggct tctgccaggt ggtgatctcc tccaacttgc gtgatggcgt
     6181 gtcccatctg atccagtccg ggggcctcgg ggggctgcag cacaacactg tgcttgttgg
     6241 ctggccccgc aactggcgcc agaaggaaga tcatcagacg tggaggaact tcattgagct
     6301 ggtccgggaa accacagctg gccacttagc cctgctggtc accaagaacg tttccatgtt
     6361 tcctgggaac cctgagcgct tctctgaggg cagcatcgac gtttggtgga ttgtgcacga
     6421 tggaggcatg ctcatgctgc tgcccttcct gctgcggcac cacaaggtct ggcggaagtg
     6481 caagatgcgt atcttcactg tggcccagat ggatgacaat agcatccaga tgaagaagga
     6541 tctgaccaca tttctgtatc atttacgcat cactgcggag gtcgaggtgg tggagatgca
     6601 tgagagcgac atctcagctt acacctatga gaagacgttg gtgatggagc agcgttccca
     6661 gatcctcaaa cagatgcatt taaccaagaa tgagcgggag cgggagatcc agagtatcac
     6721 agatgagtca cgaggctcaa tccggagaaa gaatccagcc aacacgcggc tccgcctgaa
     6781 cgtcccagaa gagacggctg gtgacagtga agagaagcca gaggaggagg tgcagctgat
     6841 ccacgatcag agtgctccca gctgccccag cagctccccg tccccagggg aggagcctga
     6901 gggggaaggg gagacagatc cggagaaggt gcatctcacc tggaccaagg acaagtcggt
     6961 ggcagagaag aataagggcc ccagtcctgt ctcctctgag ggcatcaagg acttcttcag
     7021 catgaagccg gagtgggaga acttgaacca gtccaacgtg cggcgcatgc acacggccgt
     7081 gcggctgaac gaggtcatcg tgaagaaatc ccgggacgcc aagcttgttt tgctcaacat
     7141 gcctgggcct ccccgcaacc gcaatggtga tgaaaactac atggagtttc tcgaggtcct
     7201 cacagagcac ctggaccggg tgatgctggt ccgcggcggc ggccgcgagg tcatcaccat
     7261 ctactcctga gaaccaggtc taggggatcc atacccatac gatgttccag attacgcata
     7321 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     7381 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     7441 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     7501 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     7561 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     7621 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     7681 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     7741 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     7801 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     7861 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggatcgc tagctgccgc
     7921 caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
     7981 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     8041 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     8101 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     8161 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     8221 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     8281 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     8341 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     8401 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     8461 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     8521 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     8581 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     8641 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
     8701 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     8761 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     8821 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     8881 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     8941 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     9001 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     9061 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     9121 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccgct acctggtgga
     9181 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     9241 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     9301 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca aggactataa
     9361 ggacgatgat gacaagtagc ggccgcacgc gtcaagggcg aattggccgc gactctagag
     9421 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9481 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9541 agcttataat gggtacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9601 ttcactgcct cgacatgcat gggtgcggcg agcggtatca gctcactcaa aggcggtaat
     9661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccag
//
