LOCUS       sequence-122773-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag tagged mammalian expression of E domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Tyagarajan SK, Ghosh H, Yevenes GE, Imanishi SY, Zeilhofer HU, 
            Gerrits B, Fritschy JM
  TITLE     Extracellular signal-regulated kinase and glycogen synthase kinase 
            3beta regulate gephyrin postsynaptic aggregation and GABAergic 
            synaptic function in a calpain-dependent mechanism.
  JOURNAL   J Biol Chem. 2013 Apr 5;288(14):9634-47. doi: 
            10.1074/jbc.M112.442616. Epub 2013 Feb 13.
  PUBMED    23408424
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.442616"; journalName: "J Biol Chem"; date: 
            "2013-04-5- 5"; volume: "288"; issue: "14"; pages: "9634-47"
COMMENT     Sequence Label: pCR3-FLAG-Gephyrin E
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             71..118
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttatg gactacaagg acgacgacga caaggactac aaggacgacg acgacaaggg
      121 agcaggagca ggggggttca ttacagttct ggagatgact ccggtgcttg gtacagaaat
      181 catcaattac cgagatggaa tggggcgagt ccttgctcaa gatgtgtatg caaaagacaa
      241 cctaccccca ttcccagcat cagtaaaaga tggctatgct gttcgagctg ctgatggtcc
      301 aggagatcgt ttcatcattg gggaatccca agctggtgaa cagccaactc agacagtaat
      361 gcccggacaa gtgatgcggg ttacaacagg tgctccaatc ccctgcggtg ctgatgcagt
      421 agtgcaagtt gaagatactg aactcatcag ggaatcagat gatggtactg aagaacttga
      481 agtgcgcatt ctggtgcaag ctcggccagg ccaagacatc agacccatcg gccacgacat
      541 taaaagaggg gagtgtgttt tggcaaaagg aacccatatg ggcccttcag aaattggtct
      601 ccttgcaact gtaggtgtca cagaagttga agttaataag tttccagtgg ttgccgttat
      661 gtcaacaggg aatgagctac taaatcctga agatgacctc ttaccaggaa agattcggga
      721 cagcaatcga tcaacacttc tagcaacaat tcaggaacat ggttacccaa caatcaactt
      781 gggaattgta ggagacaacc cagatgactt actcaatgcc ttgaatgagg gtatcagccg
      841 tgctaatgtc atcatcacgt caggaggtgt atccatgg
//
