LOCUS       sequence-122775-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: RBS-RFP(ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_8_RBS34- mCherry -B0015
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(441..447,448..465,466..471))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    486..507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(627..644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccagatatct ttctagaaga tctcctacaa tattctcagc tgccatggaa aatcgatgtt
       61 cttcttttat tctctcaaga ttttcaggct gtatattaaa acttatatta agaactatgc
      121 taaccacctc atcaggaacc gttgtaggtg gcgtgggttt tcttggcaat cgactctcat
      181 gaaaactacg agctaaatat tcaatatgtt cctcttgacc aactttattc tgcatttttt
      241 ttgaacgagg tttagagcaa gcttcaggaa actgagacag gaattttatt aaaaatttaa
      301 attttgaaga aagttcaggg ttaatagcat ccattttttg ctttgcaagt tcctcagcat
      361 tcttaacaaa agacgtctct tttgacatgt ttaaagttta aacctcctgt gtgaaattat
      421 tatccgctca taattccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      481 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgccaat tgctttccag
      541 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      601 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaa
//
