LOCUS       sequence-122777-         563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 563)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: px330_PTEN exon 5 gRNA
FEATURES             Location/Qualifiers
     source          1..563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        34..109
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        206..491
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 aggacgaaac accgcagcaa ttcactgtaa agcgttttag agctagaaat agcaagttaa
       61 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt
      121 agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct
      181 gcagacaaat ggctctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt
      301 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      361 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg
      421 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      481 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      541 ccccaccccc aattttgtat tta
//
