LOCUS       sequence-122780-         286 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 286)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: px330_SMAD exon 2 gRNA
FEATURES             Location/Qualifiers
     source          1..286
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        159..234
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
       61 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      121 tggaaaggac gaaacaccga aggagaaaaa agatgaatgt tttagagcta gaaatagcaa
      181 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      241 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttt
//
