LOCUS       sequence-122781-         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: px330_P53 exon 7 gRNA
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        207..282
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        379..664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccgtgc aacagctcct gcatgggttt tagagctaga aatagcaagt taaaataagg
      241 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt tttagagcta
      301 gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca
      361 aatggctcta gaggtacccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      421 caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc attgacgtca
      481 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      541 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt gtgcccagta
      601 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      661 catggtc
//
