LOCUS       sequence-122789-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CUC2 expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Rubio-Somoza I, Zhou CM, Confraria A, Martinho C, von Born P, 
            Baena-Gonzalez E, Wang JW, Weigel D
  TITLE     Temporal control of leaf complexity by miRNA-regulated licensing of 
            protein complexes.
  JOURNAL   Curr Biol. 2014 Nov 17;24(22):2714-9. doi: 
            10.1016/j.cub.2014.09.058. Epub 2014 Nov 6.
  PUBMED    25448000
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.09.058"; journalName: "Curr Biol"; date: 
            "2014-11-17- 17"; volume: "24"; issue: "22"; pages: "2714-9"
COMMENT     Sequence Label: JW616
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             89..430
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     436..455
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        436..454
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      461..470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             473..499
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atcaactcca agctttgcaa agatggataa agcggaatta attcccgagc ctccaaaaaa
       61 gaagagaaag gtcgaattgg gtaccgccgc caattttaat caaagtggga atattgctga
      121 tagctcattg tccttcactt tcactaacag tagcaacggt ccgaacctca taacaactca
      181 aacaaattct caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat
      241 gaataatgaa atcacggcta gtaaaattga tgatggtaat aattcaaaac cactgtcacc
      301 tggttggacg gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac
      361 cactacaatg gatgatgtat ataactatct attcgatgat gaagataccc caccaaaccc
      421 aaaaaaagag atctttaata cgactcacta tagggcgagc gccgccatgg agtacccata
      481 cgacgtacca gattacgctc atatggccat ggaggccagt gaattcatgg acattccgta
      541 ttaccactac gaccatggcg gagacagcca atatcttcca ccgggtttca ggtttcatcc
      601 cacggacgaa gagctcatca ctcattacct tctccgcaaa gtcctcgacg gttgcttctc
      661 aagccgtgcc atcgcagaag ttgatctcaa caagtgtgag ccttggcaac ttcccgggag
      721 agctaagatg ggagagaaag aatggtactt ctttagcctc cgtgaccgga agtatccgac
      781 gggactgaga actaacagag caactgaggc tggttactgg aaagctaccg gaaaagacag
      841 agagatcttt agttcaaaga cttgtgcact tgttgggatg aagaagactc ttgtctttta
      901 caaaggaaga gctccgaaag gagagaagag taattgggtt atgcatgaat atcgtcttga
      961 aggcaaattc tcttaccatt tcatctcaag aagctccaag gatgaatggg tgatctctag
     1021 ggttttccag aaaaccactt tagctagcac cggagccgtc tccgaag
//
