LOCUS       sequence-122819-       11060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent 
            protein), expressed in the neurons, for monitoring autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Chapin HA, Okada M, Merz AJ, Miller DL
  TITLE     Tissue-specific autophagy responses to aging and stress in C. 
            elegans.
  JOURNAL   Aging (Albany NY). 2015 Jun 30.
  PUBMED    26142908
REFERENCE   2  (bases 1 to 11060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            (Albany NY). 2015 Jun 30."
COMMENT     Sequence Label: pRab-3::Cerulean-Venus::Lgg-1
FEATURES             Location/Qualifiers
     source          1..11060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..3850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5916..5936
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7147..7171
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8002..8022
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(8774..8798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9528..9548)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9556..9573)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9556..9572)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttgatct
     5941 tcagatggga gcagtggact gtctatttga tattcttaaa atgtactcta taaaagatga
     6001 aactgtaatg accaattccc tacataaata tttttaacta attggtcgga tatttgggga
     6061 tcagagaagt acttgttttt gcgatattaa taatgatata tttttctagt aaaaaaatgt
     6121 taagcgattt gtgaataatt aagttggctt gaaaaagatt ttttgatagc tatgtatttt
     6181 gttaatttaa atcagatttt aaaactttat tattgactat ttatatatat tatatattat
     6241 atattttcat ggtttaatca aaatctatta aattatttct aaaatctcta aatatcgaat
     6301 tctctaaaaa gcttcatcaa caaaagcatt tcaattatct aacacaatga gaataattca
     6361 agaactgaaa tagaaagaaa tctagaaaaa aaagagagtg aggtgcccta ttaaatcggt
     6421 gctctcctct ctaacagaag cgcctacacg cacccaagag agctcttttt ctttcttttt
     6481 tcggagcaca catacacgca tacactcctc agaagtgtat agagaaaatg ggagaagaag
     6541 cgaaagaaga cgaagaagcg gttgtgatgt atgcatcggg cgtttctatc tctctccgtg
     6601 agcaacgagc tagtcaaccc atcgatccat ttttgtgagc acacacagag agagactcaa
     6661 acacactcac agcttcacac tgagctccaa gagctctgta gagagaagcc agagtatgct
     6721 ttcctcgatt ttcctaacgt cttcttcttc tgcttttttc tttttcccaa cgagttgtag
     6781 tccactaact tcaatttttc ctccagagaa agagttttag agagacagct gataaataca
     6841 ctagagaaat aaaaagaatg ataaaaattt gctattttaa tcagttccct ctcgtttctc
     6901 taagttttgt tgttgggttg ggaaattaaa attggaaaat ggaaataacg tttttttctg
     6961 tctaatcttg aattctccag acatttctta tttccttttt tgttgcaagt ttttcttcct
     7021 tctctaattt ttaatcaaag atatctcgct atgaactttg aatttttgat attttgattc
     7081 tataaataca taaagtaatc taaatgctct tttaaaataa atctacagta gccctatttt
     7141 cagttgacaa gtttgtacaa aaaagcaggc tcaatggtgt caaagggcga agagctgttt
     7201 actggagtcg tcccaattct ggtggagctg gatggagatg tcaacgggca taagttttca
     7261 gtgcgaggag agggagaagg agacgctacc aacggcaagc tgacactgaa attcatcagc
     7321 accacaggga agctgcccgt gccttgggta agtttaaaca tatatatact aactaaccct
     7381 gattatttaa attttcagcc aaccctggtc actaccctga gttggggcgt ccagtcattt
     7441 gcccgatacc cagaccacat gaagcagcat gatttcttta aatccgctat gcccgagggc
     7501 tacgtgcagg aacggacaat tttctttaag gacgatggaa catacaaaac tagtaagttt
     7561 aaacagttcg gtactaacta accatacata tttaaatttt caggagcaga ggtgaagttc
     7621 gaaggagaca ctctggtcaa caggatcgag ctgaagggca ttgactttaa agaagatgga
     7681 aatatcctgg gccacaaact ggagtacaac gccatccatg gcaacgtgta cattacagct
     7741 gataagcaga aaaacgggat taaggcaaat ttcggactga actgcaatgt ggaagacggg
     7801 agtgtccagc tggccgatca ctaccagcag aacactccta tcggcgacgg gcccgtgctg
     7861 ctgcctgata atcattatct gagcacccag tccgtcctgt ctaaggaccc caatgagaaa
     7921 agagatcaca tggtgctgct ggagtttgtg accgctgctg gcattaccct ggggatggac
     7981 gagctgtaca agggaccagg cgagaatttg tattttcagg gtcgtcttgt caagttcctt
     8041 gttggaggac caggcatggt gtctaaaggc gaggaactgt tcactggggt ggtgcccatc
     8101 ctggtggagc tggacggcga tgtgaatggg cacaagttta gcgtgcgcgg agagggagaa
     8161 ggggacgcta ccaacggaaa gctgacactg aaactgattt ccaccacagg caagctgccc
     8221 gtgccttggc caacactggt gactaccctg ggatacggcc tccagagctt cgcccggtat
     8281 ccagaccaca tgaagcagca tgatttcttt aaatcagcta tgcccgaggg ctacgtgcag
     8341 gaacggacaa tcttctttaa ggacgatggg acatataaaa ctagagccga ggtgaagttt
     8401 gaaggggaca ctctggtgaa caggatcgag ctgaagggaa ttgacttcaa agaagatgga
     8461 aatatcctgg gccacaagct ggagtacaac ttcaacagcc ataacgtgta tatcaccgcc
     8521 gataagcaga aaaacggcat caaggctaat ttcaaaatta ggcacaatgt ggaagacggc
     8581 ggggtgcagc tggctgatca ttaccagcag aacacaccta tcggggacgg acccgtgctg
     8641 ctgcctgata atcactacct gagctatcag tccgtgctgt ctaaggaccc aaacgagaaa
     8701 cgggatcata tggtgctgaa agaatttgtg actgccgctg ggattaccct gggaatggat
     8761 gagctgtata agcacccagc tttcttgtac aaagtgggaa tgaagtgggc ttacaaggag
     8821 gagaacaact ttgagaagcg tcgtgccgaa ggagacaaga tccgcagaaa gtacccagac
     8881 cgtattccag tgattgttga gaaagcacca aagtcaaagc tccatgactt ggataagaag
     8941 aagtacttgg tcccatccga tcttactgtt ggacagttct acttcctcat cagaaaacgc
     9001 atccaacttc gtccagaaga tgctctgttc ttctttgtca acaatgtcat tccacaaacc
     9061 atgaccacaa tgggacaact ctaccaggta actaaccagt cgtttatttt catttaatta
     9121 acccttttct ttattacagg accatcacga ggaagacttg ttcctttaca tcgcctacag
     9181 tgacgaaagt gtgtatggag gagaggtcga aaagaaggaa taaagtgtca tgtattatcc
     9241 gccgacgaat gtgtatactt gtacaatgga aatttcatac ttctaatgga aacccaaagc
     9301 ccctttaaaa aaatcttctc attttctgta ataattctgt atttccttca cattttcttc
     9361 tcatttttct cgtatgacgg ttccgagtag tgacttctat tcaaactatt gtctacgaag
     9421 ttgctcttct cccctcccag aaaatcatgt tttctctcgg attttgatat ttttttgcta
     9481 ttactttcaa atttttgatt catgaataaa cgaagaagac acaaaaccaa ctttattata
     9541 catagttgat aattcactgg ccgtcgtttt acactcgaga cgtacggtgc gcgcgatgca
     9601 ttcgaagatc tgcccactag tgagtcgtat tataagtgca agtaagatca gtgtttgttt
     9661 cgatatcagt ctgtttcgta acggtcttct gtataactac aaaaaagaat aaaaaaccgt
     9721 atcacaactg agaaaaaaga caaaacttat tgaacagtgt tcctttcaaa aaattatttt
     9781 gtaaaaaatc gagtttttct ccaaaataac gcacgcaatc aaaaacaaga aaactaacgt
     9841 gttctcctgg gcgtggaact ccttatcaag attaccatta ttgtctgtca gaattgatat
     9901 ttcaattatt tcatatctag acaaacccgg gtgtcttgca tttattagta ctgtgagttt
     9961 acatacaaac gaggcttact cagataacat agagtcacct ctaattagca aaaacccatt
    10021 tctaattagc tatttcgacc agttctaaag tcaccgtaat tagaaatggt ttttgtagct
    10081 tttctaatta gaaatgaccc cgagataagt gaaatatggc attttctggg ttttctagta
    10141 agaaatgatc ccgcacgtag tgattccgcg ttacaagcca aattcaaatt aaaaaagtat
    10201 attacacaat tcaagttaca ttattttcag gaaacaatgg cgaagagcaa agcagagatt
    10261 caaaaggagg aaagagacaa cagatatgaa cgaagacttc ttgaaaagag aaagagacat
    10321 gaggtgagta accatgataa taaataatca agtaagggct atagatttat aaattctgga
    10381 gttaacagtt tcgaaaaaaa gtagttttca tatgtgcgaa ggaatatcaa aaatagggga
    10441 atagcctaaa cgagtgagaa aattaaatgt ttgttatttt ttcaaaaatg tctgcagggc
    10501 cgttatcctt tcaaatgcac tctatcaaac atagatcata tccatcatat cttatccaga
    10561 atttatatca tatccaagat tgagagcgtt tcaaggaaaa tctcaaacaa ccatacaatt
    10621 ttgtagatcg acaacaaggc ctccacttct tcatcgatgg cctctgcagc acctgcacca
    10681 ccaagtttgt atttttcata ttaatataaa gtgagccacg catagtcgtt tttaggattt
    10741 ttgccagatc gatgtcgcgc aaacagactg tttttttttc tttgaaagcc gtgcgtgaca
    10801 tgggccacgg attgtaacgc cgcgctggat ggataaaaat ttattcagtt ttttagcctt
    10861 tacaaaatat ggttcgacta ctttctttat ttttacattt ttataggtgc tcattgagct
    10921 gagatatttt gggtataatc ggaaaatcgc tgattttggt catttttctg cactaaagat
    10981 tacacaaaaa gttcagtatt tagattcatt gttgaaaatt ggggatgatc tacagataca
    11041 tatcgccgtg ttcaaaaaca
//
