LOCUS       sequence-122822-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent 
            protein), expressed in hypodermis, for monitoring autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Chapin HA, Okada M, Merz AJ, Miller DL
  TITLE     Tissue-specific autophagy responses to aging and stress in C. 
            elegans.
  JOURNAL   Aging (Albany NY). 2015 Jun 30.
  PUBMED    26142908
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            (Albany NY). 2015 Jun 30."
COMMENT     Sequence Label: pDpy-7::oxCerulean-oxVenus::Lgg-1
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    233..253
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    598..622
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tattttttcg ctgtcctgtc acactcgcta aaaacactaa tcacacggaa atctgttttc
       61 aataaaaact ccaactttaa ctcattttca atttcaactg aaagattttt tcattagaga
      121 atgtctagaa ctaggcccgg gctacgtaat acgactcact taaggccttg actagagggt
      181 accagagctc acctaggcag gaacagctat gaccatgatt acgccaagct atcaactttg
      241 tatagaaaag ttgtctcatt ccacgatttc tcgcaacaca tcccttatat tctacattcc
      301 tttttcgcat gtactcatct cttctcgttt ggaatctttt cttatctccg ataattgggt
      361 tattgtgtcg ataaagactg acctatttaa cagatacact ccaccttcat ccacctttcc
      421 gttttctaca atccatgttc ttttcaattc aattcattta tcacaccctg ttcttttttt
      481 taatttatat tttttgtcaa aactcgaaat attttttcta ccgctctgtg aaaacaaaac
      541 aagattcatt ctgaatgaaa caatttttaa gaatattctt acattttgtt ccagataaca
      601 agtttgtaca aaaaagcagg ctcaatggtg tcaaagggcg aagagctgtt tactggagtc
      661 gtcccaattc tggtggagct ggatggagat gtc
//
