LOCUS       sequence-122823-       10194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent 
            protein), expressed in hypodermis, for monitoring autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Chapin HA, Okada M, Merz AJ, Miller DL
  TITLE     Tissue-specific autophagy responses to aging and stress in C. 
            elegans.
  JOURNAL   Aging (Albany NY). 2015 Jun 30.
  PUBMED    26142908
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            (Albany NY). 2015 Jun 30."
COMMENT     Sequence Label: pDpy-7::oxCerulean-oxVenus::Lgg-1
FEATURES             Location/Qualifiers
     source          1..10194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..3850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5916..5936
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6281..6305
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7136..7156
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(7908..7932)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8662..8682)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8690..8707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8690..8706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttgtctc
     5941 attccacgat ttctcgcaac acatccctta tattctacat tcctttttcg catgtactca
     6001 tctcttctcg tttggaatct tttcttatct ccgataattg ggttattgtg tcgataaaga
     6061 ctgacctatt taacagatac actccacctt catccacctt tccgttttct acaatccatg
     6121 ttcttttcaa ttcaattcat ttatcacacc ctgttctttt ttttaattta tattttttgt
     6181 caaaactcga aatatttttt ctaccgctct gtgaaaacaa aacaagattc attctgaatg
     6241 aaacaatttt taagaatatt cttacatttt gttccagata acaagtttgt acaaaaaagc
     6301 aggctcaatg gtgtcaaagg gcgaagagct gtttactgga gtcgtcccaa ttctggtgga
     6361 gctggatgga gatgtcaacg ggcataagtt ttcagtgcga ggagagggag aaggagacgc
     6421 taccaacggc aagctgacac tgaaattcat cagcaccaca gggaagctgc ccgtgccttg
     6481 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agccaaccct
     6541 ggtcactacc ctgagttggg gcgtccagtc atttgcccga tacccagacc acatgaagca
     6601 gcatgatttc tttaaatccg ctatgcccga gggctacgtg caggaacgga caattttctt
     6661 taaggacgat ggaacataca aaactagtaa gtttaaacag ttcggtacta actaaccata
     6721 catatttaaa ttttcaggag cagaggtgaa gttcgaagga gacactctgg tcaacaggat
     6781 cgagctgaag ggcattgact ttaaagaaga tggaaatatc ctgggccaca aactggagta
     6841 caacgccatc catggcaacg tgtacattac agctgataag cagaaaaacg ggattaaggc
     6901 aaatttcgga ctgaactgca atgtggaaga cgggagtgtc cagctggccg atcactacca
     6961 gcagaacact cctatcggcg acgggcccgt gctgctgcct gataatcatt atctgagcac
     7021 ccagtccgtc ctgtctaagg accccaatga gaaaagagat cacatggtgc tgctggagtt
     7081 tgtgaccgct gctggcatta ccctggggat ggacgagctg tacaagggac caggcgagaa
     7141 tttgtatttt cagggtcgtc ttgtcaagtt ccttgttgga ggaccaggca tggtgtctaa
     7201 aggcgaggaa ctgttcactg gggtggtgcc catcctggtg gagctggacg gcgatgtgaa
     7261 tgggcacaag tttagcgtgc gcggagaggg agaaggggac gctaccaacg gaaagctgac
     7321 actgaaactg atttccacca caggcaagct gcccgtgcct tggccaacac tggtgactac
     7381 cctgggatac ggcctccaga gcttcgcccg gtatccagac cacatgaagc agcatgattt
     7441 ctttaaatca gctatgcccg agggctacgt gcaggaacgg acaatcttct ttaaggacga
     7501 tgggacatat aaaactagag ccgaggtgaa gtttgaaggg gacactctgg tgaacaggat
     7561 cgagctgaag ggaattgact tcaaagaaga tggaaatatc ctgggccaca agctggagta
     7621 caacttcaac agccataacg tgtatatcac cgccgataag cagaaaaacg gcatcaaggc
     7681 taatttcaaa attaggcaca atgtggaaga cggcggggtg cagctggctg atcattacca
     7741 gcagaacaca cctatcgggg acggacccgt gctgctgcct gataatcact acctgagcta
     7801 tcagtccgtg ctgtctaagg acccaaacga gaaacgggat catatggtgc tgaaagaatt
     7861 tgtgactgcc gctgggatta ccctgggaat ggatgagctg tataagcacc cagctttctt
     7921 gtacaaagtg ggaatgaagt gggcttacaa ggaggagaac aactttgaga agcgtcgtgc
     7981 cgaaggagac aagatccgca gaaagtaccc agaccgtatt ccagtgattg ttgagaaagc
     8041 accaaagtca aagctccatg acttggataa gaagaagtac ttggtcccat ccgatcttac
     8101 tgttggacag ttctacttcc tcatcagaaa acgcatccaa cttcgtccag aagatgctct
     8161 gttcttcttt gtcaacaatg tcattccaca aaccatgacc acaatgggac aactctacca
     8221 ggtaactaac cagtcgttta ttttcattta attaaccctt ttctttatta caggaccatc
     8281 acgaggaaga cttgttcctt tacatcgcct acagtgacga aagtgtgtat ggaggagagg
     8341 tcgaaaagaa ggaataaagt gtcatgtatt atccgccgac gaatgtgtat acttgtacaa
     8401 tggaaatttc atacttctaa tggaaaccca aagccccttt aaaaaaatct tctcattttc
     8461 tgtaataatt ctgtatttcc ttcacatttt cttctcattt ttctcgtatg acggttccga
     8521 gtagtgactt ctattcaaac tattgtctac gaagttgctc ttctcccctc ccagaaaatc
     8581 atgttttctc tcggattttg atattttttt gctattactt tcaaattttt gattcatgaa
     8641 taaacgaaga agacacaaaa ccaactttat tatacatagt tgataattca ctggccgtcg
     8701 ttttacactc gagacgtacg gtgcgcgcga tgcattcgaa gatctgccca ctagtgagtc
     8761 gtattataag tgcaagtaag atcagtgttt gtttcgatat cagtctgttt cgtaacggtc
     8821 ttctgtataa ctacaaaaaa gaataaaaaa ccgtatcaca actgagaaaa aagacaaaac
     8881 ttattgaaca gtgttccttt caaaaaatta ttttgtaaaa aatcgagttt ttctccaaaa
     8941 taacgcacgc aatcaaaaac aagaaaacta acgtgttctc ctgggcgtgg aactccttat
     9001 caagattacc attattgtct gtcagaattg atatttcaat tatttcatat ctagacaaac
     9061 ccgggtgtct tgcatttatt agtactgtga gtttacatac aaacgaggct tactcagata
     9121 acatagagtc acctctaatt agcaaaaacc catttctaat tagctatttc gaccagttct
     9181 aaagtcaccg taattagaaa tggtttttgt agcttttcta attagaaatg accccgagat
     9241 aagtgaaata tggcattttc tgggttttct agtaagaaat gatcccgcac gtagtgattc
     9301 cgcgttacaa gccaaattca aattaaaaaa gtatattaca caattcaagt tacattattt
     9361 tcaggaaaca atggcgaaga gcaaagcaga gattcaaaag gaggaaagag acaacagata
     9421 tgaacgaaga cttcttgaaa agagaaagag acatgaggtg agtaaccatg ataataaata
     9481 atcaagtaag ggctatagat ttataaattc tggagttaac agtttcgaaa aaaagtagtt
     9541 ttcatatgtg cgaaggaata tcaaaaatag gggaatagcc taaacgagtg agaaaattaa
     9601 atgtttgtta ttttttcaaa aatgtctgca gggccgttat cctttcaaat gcactctatc
     9661 aaacatagat catatccatc atatcttatc cagaatttat atcatatcca agattgagag
     9721 cgtttcaagg aaaatctcaa acaaccatac aattttgtag atcgacaaca aggcctccac
     9781 ttcttcatcg atggcctctg cagcacctgc accaccaagt ttgtattttt catattaata
     9841 taaagtgagc cacgcatagt cgtttttagg atttttgcca gatcgatgtc gcgcaaacag
     9901 actgtttttt tttctttgaa agccgtgcgt gacatgggcc acggattgta acgccgcgct
     9961 ggatggataa aaatttattc agttttttag cctttacaaa atatggttcg actactttct
    10021 ttatttttac atttttatag gtgctcattg agctgagata ttttgggtat aatcggaaaa
    10081 tcgctgattt tggtcatttt tctgcactaa agattacaca aaaagttcag tatttagatt
    10141 cattgttgaa aattggggat gatctacaga tacatatcgc cgtgttcaaa aaca
//
