LOCUS       sequence-122834-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  -The prostate specific promoter controls expression of FlpO linked 
            to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 
            SMAD4 ex2. are expressed by the U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2,...
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(477..552)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(631..650)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 atctggacct cactgacaag gtgcagagta gcttcctgct ttttatagct gcttttcagg
       61 atgattggca tgggcagtgt cttgcatccc agttggcagt tgtacacaga cattgacatt
      121 gtgtcatcta gtatacaaat aggttcttgg agtactttac taggcatgga caatgcccaa
      181 tgcctgtccc attcttcagg catattttta tttgtgggct ttatgtccct attaagaaaa
      241 agactaagaa caagatgcta tcatattttc ttaactggaa tggtagatgt ttaaacatga
      301 tgactattac attgtgtcat ctagtataca aataggttct tggagtactt tactaggcat
      361 ggacaatgcc caatgcctgt cccattcttc aggcatattt ttatttgtgg gctttatgtc
      421 cctattaaga aaaagactaa gaacaagatg ctatcataac tagtgagctc aaaaaagcac
      481 cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc
      541 tagctctaaa acccatgcag gagctgttgc acggtgtttc gtcctttcca caagatatat
      601 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
      661 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
      721 actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat
      781 cgtatatgca aatatgaagg aatcatgg
//
